The effects of helper T-cell clone supernatants on resting human B cells were investigated. Four different helper T-cell clones (two T4+ and two T8+) were stimulated by anti-T3 monoclonal antibodies on Sepharose beads or anti-T11(2) plus anti-T11(3) monoclonal antibodies. The supernatants from these activated clones induced the proliferation of highly purified resting B lymphocytes from the per...

The progression from pre-diabetes to overt type 2 diabetes is largely attributed β-cell dysfunction and reduced insulin responsiveness. Exercise improves function in diabetics, however pre-diabetic populations there no data support a similar response acute high intensity exercise. Nine individuals diagnosed with prediabetes [HbA1c; 6.1 (0.2)%)] underwent resting control, continuous exercise hig...

Background: The present study was conducted to quantify the relationships between bone drilling process parameters(i.e., feed rate, resting time, exit rate, and drill bit diameter) and drilling outcome parameters (i.e., thrust force andmaximum temperature).Methods: This study utilized 10-cm cortical bovine samples to evaluate the effects of four independent parameters,in...

Background: Progesterone is an endogenous immunomodulator that suppresses T cell activation during pregnancy. The stimulation of membrane progesterone receptors (mPRs) would seem to be the cause of rapid non-genomic responses in human peripheral T cells, such as an elevation of intracellular calcium ([Ca2+] i) and decreased intracellular pH (pHi). Mifepristoneimmune cells compared with progeste...

A 21 year old man was referred by his general practitioner for an ultrasound evaluation of bilateral testicular masses. The masses were insidious in onset, slow growing, and palpable. There were no other local signs. The patient was also under the care of an endocrinologist for congenital adrenal hyperplasia. His current therapy included hormone replacement with oral steroids. However the patie...

Efrotomycin, a modified polyketide antibiotic with utility as a growth permittant in the animal industry, is produced by Nocardia lactamdurans. A resting cell system has been developed to facilitate biosynthetic studies. Washed cells harvested from oil-based medium at 64 hours and resuspended in buffer produce up to 700 mg/ml efrotomycin in 60 hours when supplemented with a carbon source, optim...

T cell monitoring is increasingly performed using cryopreserved PBMC. It has been suggested that resting of PBMC after thawing, that is, culturing them overnight in test medium, produces higher antigen-induced spot counts in ELISPOT assays. To evaluate the importance of overnight resting, we systematically tested cryopreserved PBMC from 25 healthy donors. CEF peptides (comprising CMV, EBV and f...