Cysteine is the preferred reducing agent used in the preparation of culture media for the growth of many strictly anaerobic microorganisms; however, redox potential reduction of cysteine is very slow, making it inconvenient if the medium is needed immediately or in large quantity. The time required to reduce culture medium containing resazurin (an indicator of reducing conditions) was dramatica...

..................................................................................................................... vii LIST OF FIGURES ...........................................................................................................xv LIST OF ABBREVIATIONS ......................................................................................... xvii CHAPTER ONE: PERSISTENT METAL CO...

Recently, we introduced a new electrochemical imaging technique called fluorescence-enabled electrochemical microscopy (FFEM). The central idea of FEEM is that a closed bipolar electrode is utilized to electrically couple a redox reaction of interest to a complementary fluorogenic reaction converting an electrochemical signal into a fluorescent signal. This simple strategy enables one to use fl...

An enzyme-catalyzed microfluidic assay using magnetic micro-beads is described. Here, diaphorase (DI) (E.C. 1.6.99) is covalently attached to the magnetic micro-beads (2.7 mum) and integrated into a short section of a microchip fabricated from PDMS. DI converts non-fluorescent resazurin to fluorescent resorufin in the presence of nicotinamide adenine dinucleotide phosphate (NADH). In this work,...

OBJECTIVES To evaluate the performance of three rapid low-cost methods for the detection of resistance to first-line drugs in Mycobacterium tuberculosis. METHODS One hundred M. tuberculosis clinical isolates were tested by the nitrate reductase assay (NRA), the MTT test and the resazurin microtitre assay (REMA), and the results compared with those obtained with the gold standard proportion me...

this study was carried out to evaluate the antimicrobial and antioxidant effectiveness of methanolic extract and different fractions (n-butanol, ethyl acetate, chloroform and n-hexane) of c.intybus seeds. the antimicrobial activity was determined by the disc diffusion method and minimum inhibitory concentration (mic) against a panel of microorganisms (four bacterial strains, i.e. p. multocida, ...

The bacterial diversity associated with biofilm-forming ability was studied. Eighteen bacterial strains were isolated from a microbial film collected from the roof of an old house located in Sfax, Tunisia. The purity of these microorganisms was confirmed by microscopic observation after repeated streaking on a Tryptic Soy agar medium. Biofilm formation was estimated using preliminary tests incl...

Nowadays, there are a number of colorimetric techniques available for the determination of a time killing assay in a manner much easier and faster than those previously more commonly used, which were much more time-consuming and laborious colony counting procedures. Here, an attempt has been made to test the antimicrobial peptides of Citropin 1.1, Palm-KK-NH2, and Temporin A on a reference stra...

We evaluated the feasibility of resazurin microtiter assay (REMA) for detecting cycloserine resistance of Mycobacterium tuberculosis. The cutoff value of the critical concentration of cycloserine resistance in vitro about M. tuberculosis isolates was determined innovatively by drawing the receiver operating characteristic (ROC) curve for the first time. The spending time of REMA ranged from 6 t...