The present study was conducted to induce callus from different in vitro grown seedling explants of shoot, root and leaf segments in order to develop shoot buds and rooting from callus. The sterilized seeds were cultured on Murashige and Skoog medium, and then the explants were cultured from seedling and transferred to an MS medium supplemented with different concentrations of BAP, kinetin, NAA...

Direct regenerations by using mature cotyledonary node as a explants has been shown to be time-saving and convenient strategy for micropropagation of soybean. So we have evaluated regeneration protocol through single shoot using cotyledonary node as a rapid and efficient protocol for two soybean cultivars and one mutant line. Cotyledonary nodes explants obtained from 7-days-old in vitro</em...

Background: Worldwidely cultivated, melon is commercially an important fruit crop, as it is in Iran. Objectives: Establishment of an efficient in vitro plant regeneration system plays a pivotal role in the plant transformation, hence, the importance of regeneration protocol for Iranian melon (Cucumis melo L. var. Gorgab) has encouraged us to work on in vitro melon regeneration. Material and Me...

the present study reports an efficient protocol for isolation and regeneration of protoplasts from callus of fritillaria imperialis l. there is no published method recommended for protoplast isolation and regeneration from fritillaria imperialis l. a range of factors, which influence the success of isolation and regeneration of f. imperialis protoplasts, were investigated. from the results obta...

Nodal explants of Phyla nodiflora (L.) Greene, were cultured on Murashige and Skoog (MS) medium supplemented with different combination and concentration of cytokinins and auxins for multiple shoots regeneration. The maximum numbers of shoots were found in MS medium supplemented with2.5 mgl-16-benzylaminopurine (BA) and 0.5 mgl-1 kinetin (KN). Elongated shoots were transferred to rooting ...

objective: n present study effect of various factors including sucrose, agar, peg, agno3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties dom siah and nemat. methods: 330 explant (mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction murashige and skoog (ms) medium (murashige and skoog 1962) s...

Objective: n present study effect of various factors including sucrose, agar, PEG, AgNO3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties Dom siah and Nemat. Methods: 330 explant (Mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction Murashige and Skoog (MS) medium (Murashige and Sk...

The gerbera market would benefit from an efficient and simple protocol for high rate regeneration for propagation and genetic engineering. With these objectives, this investigation was done on shoot regeneration via direct organogenesis from leafy petiole explants of Gerbera jamesonii ‘Royal Soft Pink’. Murashige and Skoog (1962) (MS) medium was supplemented with 0.1 mg L-1 indole-3-acetic acid...