Background: The demand for producing recombinant therapeutic proteins by mammalian cell lines, such as Chinese hamster ovary (CHO) cells, continues to grow. Significant achievements in process optimization including development of cell culture strategies for largescale and cost-effective production have been made. Fetal bovine serum (FBS) is an often essential growth supplement and yet most cos...

many findings point to the presence of a close relationship between the immune, endocrine and neurologic systems. for example the suppressive effects of β-human chorionic gonadotropin (β-hcg) on il-l production, the potentiative effect of enkephalins on lymphocyte functions and the suppressive effect of leuteinizing hormone (lh) on natural killer (nk) cell activities have been clearly establish...

OBJECTIVE Because male sex is an independent risk factor for the severity of atherosclerosis, it is possible that androgens may be proatherogenic. There is evidence that sex hormones, particularly estrogens, regulate (or modulate) inflammation, a process integral to atherogenesis. Because levels of serum inflammatory markers predict cardiovascular outcomes, we prospectively assessed the effects...

Ectopic secretion of beta-human chorionic gonadotropin is considered a poor prognostic marker in epithelial tumors. However, very few cases have been reported in sarcomas. We present the case of a 26-year-old female who presented with a metastatic osteosarcoma. She underwent usual testing prior to starting treatment and was found to have elevated levels of beta-human chorionic gonadotropin. As ...

a highly sensitive electrochemicalimmunosensor was developed to detect hcg based on immobilization ofhcg-antibody (anti-hcg) onto robust nanocomposite containing gr, chit,1-methyl-3-octyl imidazolium tetra fluoro borate ionic liquid (il)(gr-il-chit). aunps were used to immobilize hcg antibody on the modifiedelectrode. the amine groups of the antibody are covalently attached toaunps/gr-il-chit n...

background and aims: some nanoparticles can be used in immunoassays to increase sensitivity. this study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (bsa nps). materials and methods: at first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. then the concentration of β-subunit of human chorionic gonadotropin (βhcg) i...

Gonadotropin therapy plays an integral role in ovarian stimulation for infertility treatments. Efforts have been made over the last century to improve gonadotropin preparations. Undoubtedly, current gonadotropins have better quality and safety profiles as well as clinical efficacy than earlier ones. A major achievement has been introducing recombinant technology in the manufacturing processes f...