conclusions the results of this study demonstrated that the p450 cyp141 gene was successfully cloned into a pet26b plasmid vector as an expression vector. in this paper, for the first time in iran, this gene was cloned for more purposes, including the expression and purification of the recombinant cytochrome p450 cyp141 protein. results the cloning of p450 cyp141 gene was confirmed by the enzym...

background: phenylalanine dehydrogenase (phedh ec 1.4.1.20) is a nad+-dependent enzyme that performs the reversible oxidative deamination of l-phenylalanine to phenylpyruvate. it plays an important role in detection and screening of phenylketonuria (pku) diseases and production of chiral intermediates as well. the main goal of this study was to find a simple and rapid alternative method for pur...

The ecto-5′-nucleotidase CD73 is involved in the production of immunosuppressive adenosine tumoral microenvironment and recently became a validated target immuno-oncology. To avoid formation CD73-produced adenosine, several series potential inhibitors enzyme based on triazole scaffold were synthetized evaluated recombinant purified hCD73 cell-based assays.

background anaerobic-inducible promoters are alternatives of chemical-inducible promoters for expression of recombinant proteins especially in conditions where chemical induction is not possible or anaerobic conditions are preferable. the nirb promoter is the promoter of the first gene of nir operon in escherichia coli, which encodes nadh-dependent nitrite reductase. this promoter is naturally ...

background: degradation of phytic acid to inorganic phosphate in domestic animals’ diets requires thermostable phytase. although basillus subtilis phytase shows a potential to be degraded phytate complex in high temperature, the enzyme activities and yields need to be increased to make them possible for industrial application. methods: the phytase gene from bacillus subtilis dr8886 was isolated...

Benzyl Alcohol Dehydrogenase (BADH) is an important enzyme for hydrocarbon degradation, which can oxidize benzyl alcohols to aldehydes, while being capable of catalyzing a reversible reaction by reducing benzaldehyde. BADH is a member of medium chain alcohol dehydrogenases, in which zinc and NAD are essential for enzyme activity. This paper describes the expression, purification, and characteri...

Benzyl Alcohol Dehydrogenase (BADH) is an important enzyme for hydrocarbon degradation, which can oxidize benzyl alcohols to aldehydes, while being capable of catalyzing a reversible reaction by reducing benzaldehyde. BADH is a member of medium chain alcohol dehydrogenases, in which zinc and NAD are essential for enzyme activity. This paper describes the expression, purification, and characteri...

Background: Phytate is an anti-nutritional factor in plants, which catches the most phosphorus contents and some vital minerals. Therefore, Phytase is added mainly as an additive to the monogastric animals’ foods to hydrolyze phytate and increase absorption of phosphorus. Objectives: Y. intermedia phytase is a new phytase with special characteristics such as high specific activity, pH stabilit...