Real-time PCR is the state-of-the-art technique to quantify nucleic acids for mutation detection, genotyping and chimerism analysis. Since its development in the 1990s, many different assay formats have been developed and the number of real-time PCR machines of different design is continuously increasing. This review provides a survey of the instruments and assay formats available and discusses...

objectives the cause of rheumatoid arthritis (ra) as a chronic inflammatory autoimmune disease is still unknown. it appears that both genetic and environmental factors play a role in its pathogenesis. recent studies reveal that in addition to the cns, immune cells synthesis neurotransmitters so that these catecholamines can regulate immune functions. the aim of this study is to evaluate the dop...

background and aims: influenza a virus infects birds and some mammalian including human. h1 and h3 subtypes are circulated in both human and birds’ population. to determine the prevalence of the mentioned subtypes in birds, different captive bird species in tehran zoo, and affiliated centers in tehran were investigated for virus infection. methods: in this study, between november 2008 and febru...

methods allplex gi-virus assay was developed to detect pathogens causing viral gastroenteritis, one of the major diseases caused by rna viruses. this one-step multiplex real-time polymerase chain reaction (pcr) based on the mudt technique permits simultaneous amplification and detection of target nucleic acids of norovirus gi, norovirus gii, rotavirus a, adenovirus f, astrovirus, and sapovirus ...

background and objectives: hbv dna monitoring is important in management of chronic viral hepatitis b infection. hbv dna measurements are carried out over period of months to years. so the analytical system must be stable and reproducible. the aim of this study was to determine the performance characteristics and to plan a statistical quality control system of a laboratory-developed real-time q...

conclusions our results demonstrated a novel h7n9 virus in chickens in taizhou city in 2013 that may pose a potential risk to public health. results of 150 samples tested, 7 were detected to be h7n9 positive which all were collected from chickens. also, a novel h7n9 virus strain (a/chicken/jiangsu/1021/2013) was isolated and studied. phylogenetic analysis showed the novel isolated h7n9 virus ha...

background: the wilms tumor 1 (wt1) gene is originally defined as a tumor suppressor gene and a transcription factor that overexpressed in leukemic cells. it is highly expressed in more than 80% of acute myeloid leukemia (aml) patients, both in bone marrow (bm) and in peripheral blood (pb), and it is used as a powerful and independent marker of minimal residual disease (mrd);we have determined ...

vaccines is considered as a critical point and in-process of quality control (ipqc) test of vaccine production. rapid tests, like real time pcr, are more appropriated when the production occurs at industrial scale because of the amounts of starting materials and the excess of consumed time required. in the current study, a real-time quantitative reverse transcription-polymerase chain reaction (...