conclusions the viral load in patients who suffer from cchf has a direct significant correlation with disease severity. viral load above 8.6 × 105 copies/ml on the first day of admission is a predictor of severe cchf. background crimean-congo hemorrhagic fever (cchf) is an endemic disease in south eastern of iran, especially in sistan and baluchestan province. cchf is a potentially fatal diseas...

The ethiological factor of periodontal diseases is the presence periodontopathogens; in state imbalance with commensals they begin to affect pathologically. With a decrease number periodontopathogens biofilm, it possible restore balance and prevent or their transition stage remission. Nowadays, most informative accessible diagnostic method for determining real-time polymerase chain reaction (PC...

The real-time polymerase chain reaction (RT-PCR), also called quantitative real-time polymerase chain reaction (QRT-PCR) or kinetic polymerase chain reaction (kPCR), is a technique used to simultaneously quantify and amplify a DNA molecule. It is used to determine whether a specific DNA sequence is present in the sample; and if it is present, the number of copies in the sample. It is the real-t...

Real-time PCR is the state-of-the-art technique to quantify nucleic acids for mutation detection, genotyping and chimerism analysis. Since its development in the 1990s, many different assay formats have been developed and the number of real-time PCR machines of different design is continuously increasing. This review provides a survey of the instruments and assay formats available and discusses...

objectives the cause of rheumatoid arthritis (ra) as a chronic inflammatory autoimmune disease is still unknown. it appears that both genetic and environmental factors play a role in its pathogenesis. recent studies reveal that in addition to the cns, immune cells synthesis neurotransmitters so that these catecholamines can regulate immune functions. the aim of this study is to evaluate the dop...

methods allplex gi-virus assay was developed to detect pathogens causing viral gastroenteritis, one of the major diseases caused by rna viruses. this one-step multiplex real-time polymerase chain reaction (pcr) based on the mudt technique permits simultaneous amplification and detection of target nucleic acids of norovirus gi, norovirus gii, rotavirus a, adenovirus f, astrovirus, and sapovirus ...