The expression of gonadotrophin-releasing hormone receptor (GnRH-R) in germinal cells of mouse testis, whole testes, pituitary glands, and mouse ovaries was determined by means of Northern hybridization using a mouse GnRH-R [(32)P]-labelled cDNA probe. Also, the expression of GnRH-R in rat germinal cells, testis and pituitary gland was determined by Northern blot analysis using the same mouse-s...

11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) and type 2 (11β-HSD2) are expressed in rat testis, where they regulate the local concentrations of glucocorticoids. Here, we investigated the expression and localization of 11β-HSD in rat testis during postnatal development, and the regulation of these genes by luteinizing hormone (LH) and androgens. mRNA and protein levels were analyzed by qua...

background: artesunate is commonly used in malaria therapy. many antimalarial drugs have been associated with male reproductive dysfunction. the effect of artesunate on male reproductive activities was studied using in–vivo and in-vitro experimental models. methods: adult male rats (n=6) were orally given artesunate (2.9 mg/kg body weight) on daily basis for five days. artesunate (2.9 mg/kg bod...

The tissues most effective in the enzymatic hydrolysis of acyl esters of p-nitrophenol by tissue extracts of the rat and dog were the liver, lung, pancreas, renal cortex, and testis; in the dog tracheal and vesical mucosae were also esterase-rich and the lens had appreciable activity. Esterase was at low concentration in the tissues of the rat for 4 and 5 days after birth, but an increase to ad...

TRA98 is a rat monoclonal antibody (mAb) which recognizes specific antigen in the nuclei of germ cells. mAb has been used to understand mechanism cell development and differentiation many studies. In mice, recognized by or GCNA1 reported be GCNA gene product, but despite demonstration immunoreactivity this human testis sperm 1997, humans remains unknown, as date. To identify TRA98, comprehensiv...

In this study, we investigated the expression of TR4 in different stages of seminiferous tubules and the relationship between TR4 and androgen in rat testis. We found that TR4 was stage-dependently expressed in rat seminiferous tubules, T withdrawal induced by high doses of testosterone undecanoate and ethane dimethane sulfonate inhibit TR4 expression in rat testis, and testosterone induced TR4...

A determination of the absolute amounts of high-mobility-group proteins 1 and 2 (HMG1 and HMG2) in rat tissues demonstrated that amounts of HMG2 were low in non-proliferating tissues, somewhat higher in proliferating and lymphoid tissues, but were extremely elevated in the testis. This increase was due to a germ-cell-specific form of HMG2 with increased mobility relative to somatic HMG2 on acid...

Background γ-Aminobutyric acid (GABA) is considered to be the predominant inhibitory neurotransmitter in mammalian central nervous systems (CNS). There are two major classes of GABA receptors: GABAARs and GABABRs. The GABAA receptor is derived from various subunits such as alpha1-alpha 6, beta1-beta 3, gamma1-gamma 4, delta, epsilon, pi, and rho1-3. Intensive research has been performed to und...