The virus neutralization (VN) test is a reliable indicator of adequate vaccination in animals. However, the VN test is tedious and complicated to perform. Enzyme-linked immunosorbent assay (ELISA), though rapid and simple compared to the VN test, is complicated and hazardous during preparation of the viral antigen. In an effort to overcome the disadvantage of ELISA, the recombinant His-tagged n...

The suggestion that antibodies might enhance rabies virus infection of macrophages through opsonization of immune complexes was tested in vitro by adaptation of the rapid fluorescent focus inhibition technique for the examination of a macrophage cell line (P388D1). Some enhancement of rabies virus infection was shown. The relationship between such enhancement with the 'early death' phenomenon a...

the focus of this study was antimicrobial membrane-activity of actinomycetes isolated from soils of iran. in this work, soil samples were collected from desert and farming zones of northern and central iran. a total number of 45 actinomycetes were isolated from the soil samples. in the primary screening performed to evaluate antimicrobial activity, isolated microorganisms were analyzed in terms...

Antibody responses to a conventional rabies preexposure regimen of a new purified Vero cell rabies vaccine (PVRV) and a human diploid cell vaccine (HDCV) were compared in 80 healthy Kenyan veterinary students. Forty-three of the students received the PVRV and 37 received the HDCV on days 0, 7, and 28. Antibody responses were monitored by using the rapid fluorescent-focus inhibition test (RFFIT)...

Assessment of rabies virus (RABV) neutralizing antibodies in subjects vaccinated or injected with anti-RABV immunoglobulins is central determination protection. The rapid fluorescent focus inhibition test (RFFIT) used for assessment activity serum. current polyclonal preparations on the market pose difficulties production and vary quality. RABV monoclonal (MAbs) are being evaluated as replaceme...

The Anigen Rapid Rabies Antigen Test Kit (Bionote, Inc, Hwaseong, Korea) was evaluated using 80 clinical samples collected by US military veterinary units. Samples for the study were obtained from brain specimens of domestic and wildlife animals that were submitted to the US Army Public Health Command's Veterinary Laboratory Europe in Landstuhl, Germany, for rabies testing with the direct fluor...