Aiming to develop a DNA marker specific for Bacillus anthracis and able to discriminate this species from Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides, we applied the randomly amplified polymorphic DNA (RAPD) fingerprinting technique to a collection of 101 strains of the genus Bacillus, including 61 strains of the B. cereus group. An 838-bp RAPD marker (SG-850) specific for B....

Molecular characterization of Paracoccidioides brasiliensis variant strains that had been preserved under mineral oil for decades was carried out by random amplified polymorphic DNA analysis (RAPD). On P. brasiliensis variants in the transitional phase and strains with typical morphology, RAPD produced reproducible polymorphic amplification products that differentiated them. A dendrogram based ...

A diploid Solanum clone 9507-04 was used to grow anther-derived plantlets that were putatively monoploid. Sixteen RAPD primers were used to observe the RAPD profiles from anther-donor and anther-derived materials. A total of 56 RAPD markers were scored and each marker was identified according to the primer’s designation and size (kilo base pairs) of the amplified fragment. Of 56 RAPD markers as...

ABSTRACT Crown rust of barley, caused by Puccinia coronata var. hordei, occurs sporadically and sometimes may cause yield and quality reductions in the Great Plains region of the United States and Canada. The incompletely dominant resistance allele Rpc1 confers resistance to P. coronata in barley. Two generations, F(2) and F(2:3), developed from a cross between the resistant line Hor2596 (CIho ...

mahaleb (prunus mahalab l.) is used as a rootstock for cherries in iran and many other countries. in this research, rapd markers were used to determine the genetic diversity among eight genotypes of mahaleb evaluated in two separate research grardens in karaj, iran. these genotypes are used as mother plants for seed production. totally 40 random primers were examined for pcr reactions on the te...

background: garlic is a valuable medicinal plant with variability in desirable morphological and physiological characteristics. the analysis of genetic diversity plays an important role in breeding programs. the rapd technique could be very effective in detecting genetic variation in garlic.  objective: the objective of the present work was to detect molecular polymorphism among iranian garlic ...

indole acetic acid is an important hormone that improved the growth of different plant organs such as the root under normal and stress conditions. as various studies have shown, most of rhizobacteria produce indole acetic acid and thereby contribute to the growth of plants. in this study, different rhizobacteria were isolated from different regions of iran for the assessment of their ability to...

of high-quality vegetable and industrial oil. 9 rapd primers, 6 aflp primer combinations and 12 agro-morphological traits were used to assess the genetic diversity of 20 accessions of saf flower representing global germplasm variability. jacquards’ similarity coef ficient were used to understanding the genetic relationships among ccessions, for aflp and rapd markers and euclidian similarity coe...

The objective of this review is to summarize numerous studies on the use of the random amplified polymorphic DNA (RAPD) technique on rice, corn, wheat, sorghum, barley, rye, and oats to examine its feasibility and validity for assessment of genetic variation, population genetics, mapping, linkage and marker assisted selection, phylogenetic analysis, and the detection of somaclonal variation. Al...

The segregation pattern of an 810-bp random amplified polymorphic DNA (RAPD) band in the F1 and backcross generations of a Silene dioica (L.) Clairv. family provides evidence that this molecular marker is located in the pseudoautosomal region (PAR) of the X and Y chromosomes. The marker was found through a combination of bulked segregant analysis (BSA) and RAPD techniques. Recombination rates b...