We describe the application of the microencapsulated-antibody technique to the radioimmunoassay of digoxin in serum. Droplets of emulsified rabbit antibody are microencapsulated in a semipermeable nylon membrane by an interfacial polymerization technique. The antibody microcapsules are incubated with 125I-labeled digoxin and unlabeled digoxin for 15 min at 37 degrees C, then free and bound digo...

introduction: determination of thyrotropin, total and free thyroxine and triiodothyronine are widely used as diagnostic methods for thyroid function evaluation. there have been numerous reports of interferences in thyroid hormone immunoassays. herein, a prominent positive interference is reported for a radioimmunoassay kit of total triiodothyronine. materials and methods: in this study, a total...

Standard curves from 150 determinations of 10 different hormone radioimmunoassays have been evaluated using the following four procedures: logit transformation as performed by Rodbard, Healy's 4-component-logit, spline approximation and a linear interpolation program. The results, calculated by computer, have been compared with manual analysis as the refernce method. The influece of various cor...

Numerous methods are available for the graphical display of radioimmunoassay dose-response curves, for curve-fitting and dose interpolation, for statistical quality control, and for automation and computerization of data processing. The relative merits of these approaches are discussed. Minimal requirements for radioimmunoassay data-processing systems are presented. The features of an “ideal” s...

The first study about radioimmunoassay was published in 1960 by 2 scientists, who created this new method to quantify human insulin, at a time when studies on diabetes and its pathophysiology were beginning stand out. Years later, due strong relevance the scientific world, such work awarded Nobel Prize Physiology 1977. Thus, present aims expose historical context of discovery, initial diseases ...

Three methods of measuring the epidermolytic toxin Staphylococcus aureus-bioassay in newborn mice, radial immunodiffusion, and radioimmunoassay-were compared for reproducibility, specificity, and sensitivity. The bioassay is highly specific and remains the only functional assay. It is reproducible only if newborn mice of the same age are used. The time required for epidermolysis follows a dose-...

Seventy-one sera from Japanese individuals infected with human T cell leukemia virus type I (HTLV-I) were examined for the presence of antibodies to HTLV-III by an enzyme-linked immunosorbent assay (ELISA) and by a strip radioimmunoassay based on the Western blot technique. The sera were from 23 healthy carriers and from 48 patients, including 18 with smoldering adult T cell leukemia (ATL), 13 ...

Parathyrin (parathyroid hormone) radioimmunoassay is a commonly ordered endocrine assay. I discuss here of the metabolism of the hormone and the effect information on this metabolism has had on the development of radioimmunoassays for parathyrin. Although radioimmunoassay is the most widely accepted technique for the routine assay for parathyrin, "high-performance" liquid chromatography, cytoch...

A simple and rapid filter radioimmunoassay method can be used to serotype meningococcal strains on a large scale. The technique consists of simultaneous inoculation of 96 strains on nitrocellulose filters. The resulting colonies can be processed in situ, by extraction and fixation, incubation with antibodies and 125I-labeled protein A, and, finally, autoradiography. Processing many filters simu...