intranasal vaccination is particularly a striking route for mucosal immunization, due to the ease of administration and the induction of both mucosal and humoral immunity. however, soluble antigens (ag) are not sufficiently taken up after the nasal administration and need to be co-administered with adjuvants, penetration enhancers or encapsulated in particles. so, in this study, tetanus toxoid ...

In this study, a preparation of saponins (QB-90U) extracted from leaves of Quillaja brasiliensis collected in Uruguay was evaluated as a vaccine adjuvant by comparison with alum and the well known saponin-based adjuvant, Quil A. The haemolytic activity and cellular toxicity of the saponin preparations were also evaluated. QB-90U was only slightly haemolytic and showed a low cytotoxicity when co...

Quillaja brasiliensis (Quillajaceae) is a saponin producing species native from southern Brazil and Uruguay. Its saponins are remarkably similar to those of Q. saponaria, which provides most of the saponins used as immunoadjuvants in vaccines. The immunostimulating capacities of aqueous extract (AE) and purified saponin fraction (QB-90) obtained from leaves of Q. brasiliensis were favorably com...

Immune-stimulating complexes (ISCOMs) are colloidal lipidic structures particularly qualified as potent drug delivery systems for vaccine formulations. Morphologically they feature a unique cage-like structure and form spontaneously in an aqueous pseudoternary system of phospholipid, cholesterol and quillaja saponin A (Quil A). Beside the ISCOM matrices other colloidal structures can be found i...

Due to synthetic surfactants' environmental impact, their replacement by natural alternatives has gained relevance, with saponins emerging as sustainable approaches. In this work, three saponin-rich extracts from different sources (Tribulus terrestris (TT), Trigonella foenum-graecum (FG), and Ruscus aculeatus (RA)) were tested emulsifiers, performance compared Quillaja Bark saponin (PS). Charac...

A total of 3 experiments (Exp), in vitro and in vivo, were conducted to investigate the effects of saponin extracts from Quillaja saponaria (QS), Yucca schidigera (YS) and Camellia sinensis (TS) on CH4 emissions from steers. The in vitro Exp was carried out to determine the effects of saponin inclusion on ruminal methanogenesis and fermentation parameters. Two doses (0.5% and 2.0% of substrate ...

OBJECTIVES The immunoadjuvant potential of cross-linked dextran microspheres (CDM) as absorption enhancer and Quillaja saponins (QS) as immunomodulator adjuvant was evaluated. MATERIALS AND METHODS CDM loaded or tetanus-mixed toxoid (TT) or Quillaja saponin (QS) were nasally administered to rabbits in dry powder form, three times in 2 weeks interval and serum IgG and nasal lavage sIgA titers ...