D. Coman, H. K. Trubel, P. Herman, and F. Hyder Department of Diagnostic Radiology, Yale University, New Haven, CT, United States, Quantitative Neuroscience with Magnetic Resonance (QNMR), Yale University, New Haven, CT, United States, Bayer HealthCare Research Center, Wuppertal, Germany, Department of Pediatrics, HELIOS-Klinikum Wuppertal, University Witten/Herdecke, Wuppertal, Germany, Semmel...

Given the critical role of pure, organic compound primary reference standards used to characterize and certify chemical Certified Reference Materials (CRMs), it is essential that associated mass purity assessments be fit-for-purpose, represented by an appropriate uncertainty interval, and metrologically sound. The mass fraction purities (% g/g) of 25-hydroxyvitamin D (25(OH)D) reference standar...

Toxicologically relevant levels of the psychoactive ?9-tetrahydocannabinol (?9-THC) as well high non-psychoactive cannabinoids potentially occur in CBD (cannabidiol) oils. For consumer protection fast-growing oil market, facile and rapid quantitative methods to determine cannabinoid content are crucial. However, current standard method, i.e., liquid chromatography combined with tandem mass spec...

The contribution of drug metabolites to the pharmacologic and toxicologic activity of a drug can be important; however, for a variety of reasons metabolites can frequently be difficult to synthesize. To meet the need of having samples of drug metabolites for further study, we have developed biosynthetic methods coupled with quantitative NMR spectroscopy (qNMR) to generate solutions of metabolit...

Quantitative NMR (qNMR) is applied to determine the absolute quantitative value of analytical standards for HPLC-based quantification. We have previously reported optimal and reproducible sample preparation method qNMR hygroscopic reagents, such as saikosaponin a, which used an standard in assay crude drug section Japanese Pharmacopoeia (JP). In this study, we examined purity determination a su...

Synthetic cathinones are a type of new psychoactive substances (NPS) that have been seriously abused. Owing to the rapid variation in their structures, absence reference standards poses challenge quantitative investigations. In this study, 1H nuclear magnetic resonance (1H qNMR) method was established using maleic acid as internal standard and shared signal (i.e., methylidyne hydrogen) on paren...

Mogroside V is one of the characteristic and effective components luohanguo extract, a food additive used as sweetener in Japan per Japan’s Standards Specifications for Food Additives (JSFA; 9th ed.). JSFA stipulates that quantitative determination mogroside content extract applies HPLC using analytical standard V. However, no reagents with proven purities are commercially available. Therefore ...