نتایج جستجو برای: purification
تعداد نتایج: 56444 فیلتر نتایج به سال:
Introduction: Polyclonal antibodies are required to be affinity purified. Improved purification methods of polyclonal antibody provide an opportunity to pick the most purified immunoglobulins as a primary or secondary antibody in immunoassays that are included in many vaccine studies. Two common techniques for purifying proteins is salt precipitation and chromatography purification. Our work fo...
Before describing the structure and mechanism of action of a protein, it must first be subject to purification procedure. Protein purification is a set of processes in which one or a small number of proteins are purified from a complex compound that may be a complete cell, tissue, or organism. Understanding the functions, structural properties, and interactions of the protein are directly re...
Introduction Utah’s wetlands provide a constellation of recreational opportunities, from sailing to photography, fishing to birdand wildlife-watching, hunting to picnicking. Even more important are the roles wetlands play in the functioning of Utah’s ecosystems. These roles include flood control, maintenance of groundwater quality and levels, pollution control, water purification, and the provi...
introduction: scorpion venoms contain a variety of peptides, toxic to mammals، insects and crustaceans and are the main factors in scorpion venom toxicity (their amount being 1-3% of total venom). most of the scorpion toxins have been isolated from the venoms of scorpions in the buthidae family. the scorpion buthotus schach of this family is widely found in the western regions of iran, but no p...
to carry out the immunological experiments on the serum of multiple sclerosis (ms) patients, based on a correlation between borrelia burgdorferi infection and contracting ms autoimmune disease the outer surface protein d (ospd) of the bacterium was expressed and purified. a clone containing the ospd gene in pet11a expression vector under the control of t7 promoter was transformed to the bacteri...
a purification procedure of pertussis toxin (pt) from submerged culture of bordetella pertussis (b.pertussis) strain 134 using adsorption and affinity chromatography was discussed. the yield of the resulting pt was approximately 37.5mg/l of concentrated culture supernatant. the polypeptide pattern of the purified pt was investigated by sds-page and showed five bands between 11 to 26 kda using l...
pseudomonas aeruginosa as an opportunistic pathogen is a significant cause of acute and chronic infections in patients with compromised defenses. this bacterium is motile via a single polar flagellum made of polymerized flagellin subunits differentiated into two major serotypes: a and b. flagellin plays an important role as a virulence factor in the adhesion, colonization and invasion of p. aer...
mycobacterium tuberculosis (h37rv strain) was used in this study. the bacterial cells were disintegrated by sonication. the separation and characterization of the soluble molecules were attempted by various techniques including gel filtration, ion exchange chromatographies and polyacrylamide gel electrophoresis, using sds and 2me. eight protein molecules with molecular weights ranging from 6.3 ...
the aim of this study was production and partial purification of α-amylase enzyme by bacillus licheniformis. b. licheniformis was allowed to grow in broth culture for purpose of inducing α-amylase enzyme. optimal conditions for amylase production by b. licheniformis are incubation period of 120 h, temperature of 37 °c and ph 7.0. the α-amylase enzyme was purified by ion exchange chromatography ...
phenylalanine dehydrogenase (phedh; ec 1.4.1.20) is an important enzyme of amino acid dehydrogenases family that increasingly used as a valuable biocatalyst in neonatal screening kits and synthesis of l-phenylalanine. the goal of this literature was to find a suitable purification method for recombinant bacillus badius phedh by practical comparison between chromatographic and polyvinyl pyrrolid...
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