نتایج جستجو برای: purification

تعداد نتایج: 56444  

ژورنال: Vaccine Research 2016

Introduction: Polyclonal antibodies are required to be affinity purified. Improved purification methods of polyclonal antibody provide an opportunity to pick the most purified immunoglobulins as a primary or secondary antibody in immunoassays that are included in many vaccine studies. Two common techniques for purifying proteins is salt precipitation and chromatography purification. Our work fo...

  Before describing the structure and mechanism of action of a protein, it must first be subject to purification procedure. Protein purification is a set of processes in which one or a small number of proteins are purified from a complex compound that may be a complete cell, tissue, or organism. Understanding the functions, structural properties, and interactions of the protein are directly re...

2002
Dale J. Blahna

Introduction Utah’s wetlands provide a constellation of recreational opportunities, from sailing to photography, fishing to birdand wildlife-watching, hunting to picnicking. Even more important are the roles wetlands play in the functioning of Utah’s ecosystems. These roles include flood control, maintenance of groundwater quality and levels, pollution control, water purification, and the provi...

Journal: :iranian journal of toxicology 0
قانع m ghane college of science science & research unit, islamic azad university, tehran, iran زارع a zare department of venomous animals and antivenom production, razi vaccine and serum research institute, kraj, iran ربیعی h rabei department of venomous animals and antivenom production, razi vaccine and serum research institute, kraj, iran محمدپور n mohammadpour department of venomous animals and antivenom production, razi vaccine and serum research institute, kraj, iran ابراهیم حبیبی a ebrahem habibi college of science science & research unit, islamic azad university, tehran, iran

introduction: scorpion venoms contain a variety of peptides, toxic to mammals، insects and crustaceans and are the main factors in scorpion venom toxicity (their amount being 1-3% of total venom). most of the scorpion toxins have been isolated from the venoms of scorpions in the buthidae family. the scorpion buthotus schach of this family is widely found in the western regions of iran, but no p...

Journal: :archives of razi institute 2016
m.h. sanati f. alasti h. aleyasin m. mostafavi p.r. carnegie

to carry out the immunological experiments on the serum of multiple sclerosis (ms) patients, based on a correlation between borrelia burgdorferi infection and contracting ms autoimmune disease the outer surface protein d (ospd) of the bacterium was expressed and purified. a clone containing the ospd gene in pet11a expression vector under the control of t7 promoter was transformed to the bacteri...

Journal: :archives of razi institute 2016
a. pourahmadi f. esmaili a. zavaran hosseini a. rezaee e. salehi

a purification procedure of pertussis toxin (pt) from submerged culture of bordetella pertussis (b.pertussis) strain 134 using adsorption and affinity chromatography was discussed. the yield of the resulting pt was approximately 37.5mg/l of concentrated culture supernatant. the polypeptide pattern of the purified pt was investigated by sds-page and showed five bands between 11 to 26 kda using l...

Journal: :international journal of molecular and cellular medicine 0
sobhan faezi departments of mycobacteriology and pulmonary research, pasteur institute of iran, tehran, iran.سازمان اصلی تایید شده: انستیتو پاستور ایران (pasteur institute of iran) ahmad reza bahrmand departments of mycobacteriology and pulmonary research, pasteur institute of iran, tehran, iran.سازمان اصلی تایید شده: انستیتو پاستور ایران (pasteur institute of iran) mehdi mahdavi departments of immunology, pasteur institute of iran, tehran, iran.سازمان اصلی تایید شده: انستیتو پاستور ایران (pasteur institute of iran) seyed davar siadat departments of mycobacteriology and pulmonary research, pasteur institute of iran, tehran, iran.سازمان اصلی تایید شده: انستیتو پاستور ایران (pasteur institute of iran) iraj nikokar laboratory of microbiology and immunology of infectious diseases, paramedicine faculty, guilan university of medical sciences, guilan, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی گیلان (guilan university of medical sciences) soroush sardari biotechnology research center, drug design and bioinformatics group, pasteur institute of iran, tehran, iran.سازمان اصلی تایید شده: انستیتو پاستور ایران (pasteur institute of iran)

pseudomonas aeruginosa as an opportunistic pathogen is a significant cause of acute and chronic infections in patients with compromised defenses. this bacterium is motile via a single polar flagellum made of polymerized flagellin subunits differentiated into two major serotypes: a and b. flagellin plays an important role as a virulence factor in the adhesion, colonization and invasion of p. aer...

Journal: :medical journal of islamic republic of iran 0
a zavaran hosseini from the department of immunology, tarbiat modarres university mb eslami the dept. of immunology school of public health, tehran university of medical sciences, tehran, islamic republic of iran. m jalali the dept. of biochemistry, school of public health, tehran university of medical sciences, tehran, islamic republic of iran.

mycobacterium tuberculosis (h37rv strain) was used in this study. the bacterial cells were disintegrated by sonication. the separation and characterization of the soluble molecules were attempted by various techniques including gel filtration, ion exchange chromatographies and polyacrylamide gel electrophoresis, using sds and 2me. eight protein molecules with molecular weights ranging from 6.3 ...

Journal: :archives of razi institute 0

the aim of this study was production and partial purification of α-amylase enzyme by bacillus licheniformis. b. licheniformis was allowed to grow in broth culture for purpose of inducing α-amylase enzyme. optimal conditions for amylase production by b. licheniformis are incubation period of 120 h, temperature of 37 °c and ph 7.0. the α-amylase enzyme was purified by ion exchange chromatography ...

Journal: :journal of sciences, islamic republic of iran 2009
e. omidinia

phenylalanine dehydrogenase (phedh; ec 1.4.1.20) is an important enzyme of amino acid dehydrogenases family that increasingly used as a valuable biocatalyst in neonatal screening kits and synthesis of l-phenylalanine. the goal of this literature was to find a suitable purification method for recombinant bacillus badius phedh by practical comparison between chromatographic and polyvinyl pyrrolid...

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