Extremophilic fish require genetic characterization to determine the identity of their species, kinship, and potential for variation due extreme environmental adaptation. DNA barcoding using Fish-F2 Fish-R2 primers at an annealing temperature 52oC 15 seconds showed qualitative failure results in most species. For this reason, optimizing PCR process is necessary obtain bands that correlate with ...

Interspecific androgenetic rosy barb (Puntius conchonius) was generated using its cadaveric (-20 degrees C) or fresh sperm to activate nuclear genome inactivated oocytes of gray tiger barb (Puntius tetrazona). UV irradiation was used to inactivate nuclear genome of tiger barb oocytes. Thermal shock restored diploidy of rosy barb in the oocytes of tiger barb. Survival of androgenotes was 14% or ...

Haddadus binotatus is an endemic anuran of the Brazilian Atlantic Forest and currently, there is no information about the diet of this species. We analyzed the diet of two populations of this anuran in two states in southeast Brazil. Samplings were carried out in 2004 in the state of Rio de Janeiro and in 2009 and 2010 in the state of Espírito Santo. Haddadus binotatus presented a rich diet com...

Understanding the processes underlying spatial patterns of genetic diversity and structure of natural populations is a central topic in evolutionary biogeography. In this study, we combine data on ancient and contemporary landscape composition to get a comprehensive view of the factors shaping genetic variation across the populations of the scrub-legume grasshopper (Chorthippus binotatus binota...

The terminal Pleistocene and Early Holocene, a period from 15 000 to 18 000 Before Present (BP), was critical in establishing the current Holarctic fauna, with temperate-climate species largely replacing cold-adapted ones at mid-latitudes. However, the timing and nature of this process remain unclear for many taxa, a point that impacts on current and future management strategies. Here, we use a...

The water vole (Arvicola amphibius) in the forest-steppe of West Siberia is known to have wide fluctuations in abundance. These fluctuations are accompanied by changes in birth and death rates, sex-age structure of the population, and individual morphophysiological and behavioral characteristics of the animals. Survival of the animals depends on season, phase of population cycle, and sex. Based...

twenty spices were employed to preserve the cooked and uncooked fillet of puntius sarana (hamilton) and puntius ticto (hamilton). ic50 values of 2,2’-diphenyl-1-picrylhydrazyl(dpph) based free radical scavenging activity ranged from 0.1123 μg ml-1 in turmeric to 13.035 μg ml-1 in roman coriander. phenol content ranged from 0.365 μg g-1 in onion to 5.67 μg g-1 in clove. the raw and cooked fillet...

We studied the leaf-litter frog community of Estação Ecológica Estadual Paraíso, in Guapimirim, Rio de Janeiro State, southeastern Brazil. Herein we combined three sampling methods (large plots, visual encounter surveys and pit-fall traps) to present data on species composition, richness, relative abundance and densities. The local assemblage of frogs associated to the leaf-litter was composed ...

The type specimens of five species of Eleodes described by Francis Walker from British Columbia, Canada, were studied in order to establish their true identity. The synonymy of Eleodes convexicollis Walker and E. conjunctus Walker with E. obscurus (Say); that of Eleodes latiusculus Walker with E. humeralis LeConte;  and that of Eleodes binotatus Walker with  Eleodes hispilabris (Say) is confirm...