Mobility of supercoiled (form I) and nicked circular (form II) plasmid DNAs was determined on two major forms of pulsed-field electrophoresis, CHEF and OFAGE. Plasmids with molecular lengths ranging from 2.30 to 17.8 kilobase pairs (kb) were used with Saccharomyces cerevisiae chromosomes as standards. Agarose gel concentrations were varied from 0.3 to 2.0 percent, with higher percentage gels re...

Methods The usefulness of spa-typing, pulse-field gel electrophoresis (PFGE) and automated repetitive extragenic palindromic sequence-based (rep-PCR) method (DiversiLab, Bacterial Barcodes, Inc.) for typing Finnish MRSA strains was studied. A total of 110 clinical MRSA isolates collected from well-defined outbreaks in Ostrobothnia region and 96 isolates from several epidemics or sporadic source...

Laboratory technologists (22%) developed infections with Shigella sonnei. The isolates had the same antibiogram and pulse-field gel electrophoresis pattern as an unknown isolate handled by a laboratory student. Covering faucet handles with paper towels during hand washing in the laboratory was protective. No further cases occurred after the laboratory was cleaned with a phenolic agent and a han...

Ten Salmonella enterica serotypes were isolated from fecal samples collected from anesthetized raccoons (n = 738) trapped in six Pennsylvania counties from 2003 to 2005. Comparison of raccoon pulsed-field gel electrophoresis (PFGE) pulse type data with the Pennsylvania Department of Health PFGE database revealed that the patterns of seven Salmonella serotypes matched those isolated from humans ...

Background and aims: The food-borne pathogens appear to be a reemerging and endless problem inthe human community all over the world. Hence, all the outbreaks should be constantly monitored forpandemic strains and new mutant genotypes. The main purpose behind the molecular typing methodsis the comparison of bacterial isolates to obtain genomic relatedness regarding epidemiolog...

Objectives: To identify and to characterize of methicillin-resistant Staphylococcus aureus (MRSA) isolates collected from food (n=43), handler (n=8), clinical samples (n=15). Methods: Genotyping was applied for the purposes epidemiological investigation source tracking these isolates. Pulsed field gel electrophoresis (PFGE) performed using restriction endonucleases SmaI. Findings: PFGE analysis...