A radioimmunoassay specific for the amino (N) terminal portion of the parathyroid hormone (PTH) molecule (N-PTH radioimmunoassay) has been developed by iodinating synthetic 1-34bovine PTH (1-34bPTH) and using commercially available bPTH antiserum. A radioimmunoassay specific for the carboxyl (C) terminal (C-PTH radioimmunoassay) has been carried out by adding enough amount of 1-34bPTH to the PT...

Partially purified bovine parathyroid hormone (PTH) produced two kinds of antisera, GP-100 reacting with 125I-labeled bovine PTH (b-PTH 1-84) alone and GP-M reacting with 125I-labeled synthetic N-terminal portion of bovine PTH (b-PTH 1-34) as well as b-PTH 1-84. Rat serum PTH reacted with the latter but not in the former, suggesting a similarity to b-PTH 1-34. Rat serum PTH became undetectabl...

This study examined the relationships between post-traumatic headache (PTH) and mental health symptoms after concussions to inform adolescent concussion management. Headache is most common complaint following concussion. In this sample of 123 adolescents with concussion, there was a 5-fold increase in odds clinically elevated anxiety, as well increased (anxiety, depression, anger, disruptive be...

Objective To evaluate the usefulness of a third-generation PTH assay in the diagnosis of primary hyperparathyroidism (PHPT). Subjects and methods Forty-one PHPT patients (4 men and 37 women) with 61.2 ± 10.9 (mean ± SD) years, were studied and had PTH levels measured with two different methods using the same immunochemiluminescent assay plataform (Elecsys 2010 System, Roche). We compared a se...

The common intact parathyroid hormone (i-PTH) assay detects not only PTH (1-84) but also the PTH (7-84) fragment. Recently, it was reported that the PTH (7-84) fragment is an antagonist to the biological action of PTH (1-84). It was also reported that the accumulation of the PTH (7-84) fragment plays a role in skeletal resistance in haemodialysis (HD) patients. However, the role of accumulation...

AIM: Parathyroidhormone (PTH) measurement is important in the evaluation of bone disease in patients with chronic renal failure. Large carboxyl-terminal PTH fragments (C-PTH) cross-react with second-generation PTH assays, lead to an overestimation of biologically active PTH, and are evaluated by a combination of secondand third-generation PTH assays. The aim of our study was to examine whether ...

Hypocalcemia is one of the common complications after total thyroidectomy. In these patients, serum parathyroid hormone (PTH), calcium level decreases, and phosphorus increases. The fibroblast growth factor-23 (FGF-23), a phosphaturic hormone, increases in to normalize level. our study, we aimed investigate predictive values PTH, calcium, phosphorus, 1,25-dihydroxy vitamin D (vitamin D), FGF-23...

Preproparathyroid hormone (prepro-PTH) is one of the proteins abundantly synthesized by parathyroid chief cells; yet under normal growth conditions, little or no prepro-PTH can be detected in these cells. Although this may be attributed to effective cotranslational translocation and proteolytic processing, proteasome-mediated degradation of PTH precursors may be important in the regulation of t...

Despite the innovations in the treatment of secondary hyperparathyroidism, there are uremic patients with marked elevation in PTH levels. Uremic toxicity is in part attributable to the excess of circulating PTH. It has been known for many years that PTH may induce changes in cell calcium, a key intracellular signal required for normal cell function. The effect of PTH in dialysis patients is not...

Secondary hyperparathyroidism is characterized by increased parathyroid hormone (PTH) mRNA stability that leads to increased PTH mRNA and serum PTH levels. PTH gene expression is reduced by the calcimimetic R568 and the oral phosphorus binder lanthanum carbonate (La). Changes in PTH mRNA stability are regulated by the binding of trans-acting stabilizing and destabilizing factors to a defined ci...