Abstract BACKGROUND Glioblastoma (GBM) is the most aggressive and lethal type of brain tumor. Activation PI3K/mTOR pathway along with loss its primary negative regulator, phosphatase tensin homolog (PTEN), occurs in nearly 50% GBM patients. As PTEN known to promote DNA damage repair deficiency, here we investigated whether deficiency presents a vulnerability simultaneous induction suppression m...

PTEN deletion is an established prognostic biomarker in prostate cancer. We compared PTEN immunohistochemistry (IHC) and PTEN fluorescence in situ hybridization (FISH) in the largest existing radical prostatectomy cohort with clinical follow-up data. There was high concordance between IHC and FISH: 93% (3098/3330) of tumors with intact PTEN IHC showed absence of PTEN gene deletion and 66% (720/...

The balance between phosphoinositides distributed at specific sites in the plasma membrane causes polarized actin polymerization. Oncogenic transformations affect this by regulating phosphoinositide 3-kinase (PI3K) and phosphatase tensin homolog deleted on chromosome 10 (PTEN), causing metastatic behavior cancer cells. Here, we show that PTEN tumor suppressor gene is required for epithelial cel...

PARP enzyme plays a key role in the cellular machinery responsible for DNA damage repair. PTEN is a tumor-suppressor gene deactivating PI3K downstream of EGFR signaling. We hypothesize that PTEN-deficient lung cancer cells suppressed DNA damage signaling and that the absence of PTEN can sensitize these cells to a concurrent treatment of a DNA-damaging agent (cisplatin) and a PARP inhibitor (ola...

Complete inactivation of the PTEN tumor suppressor gene is extremely common in advanced cancer, including prostate cancer (CaP). However, one PTEN allele is already lost in the vast majority of CaPs at presentation. To determine the consequence of PTEN dose variations on cancer progression, we have generated by homologous recombination a hypomorphic Pten mouse mutant series with decreasing Pten...

PTEN dysfunction plays a crucial role in the pathogenesis of hereditary and sporadic cancers. Here, we show that PTEN homodimerizes and, in this active conformation, exerts lipid phosphatase activity on PtdIns(3,4,5)P3. We demonstrate that catalytically inactive cancer-associated PTEN mutants heterodimerize with wild-type PTEN and constrain its phosphatase activity in a dominant-negative manner...

background: ovarian cancer comprises a heterogeneous group of neoplasms. the prognosis cannot be predicted by histopathologic examination alone. the aim of this study is to evaluate p53, pten, and β-catenin expressions in primary ovarian carcinomas in an attempt to find a possible relationship with morphologic parameters and clinical findings. methods: the study included 100 epithelial ovarian ...

Long non?coding RNAs (lncRNAs) are involved in the occurrence and progression of numerous types cancer. The aim present study was to evaluate effect lncRNA maternally expressed gene 3 (MEG3) on migration invasion non?small cell lung cancer (NSCLC) H1299 PC9 cells. Reverse transcription?quantitative (RT?q)PCR analysis showed that MEG3 downregulated NSCLC Additionally, bioinformatics indicated sp...

PTEN (phosphatase and tensin homologue deleted on chromosome 10), a potent tumour suppressor and multifunctional signalling protein, is under intricate regulation. In the present study, we have investigated the mechanism and regulation of PTEN ubiquitination catalysed by NEDD4-1 (neural-precursor-cell-expressed, developmentally down-regulated 4-1), a ubiquitin ligase for PTEN we identified rece...