The aim of this study was to determine the changes of short chain fatty acids (SCFAs) in faeces of inflammatory bowel disease (IBD) patients compared to healthy subjects. SCFAs such as pyruvic, lactic, formic, acetic, propionic, isobutyric and butyric acids were analyzed by using high performance liquid chromatography (HPLC). This study showed that the level of acetic, 162.0 micromol/g wet faec...

We studied the effects of alcohol intake on postexercise muscle glycogen restoration with samples from vastus lateralis being collected immediately after glycogen-depleting cycling and after a set recovery period. Six well-trained cyclists undertook a study of 8-h recovery (2 meals), and another nine cyclists undertook a separate 24-h protocol (4 meals). In each study, subjects completed three ...

Nanoscale imaging techniques are needed to investigate cellular function at the level of individual proteins and to study the interaction of nanomaterials with biological systems. We imaged whole fixed cells in liquid state with a scanning transmission electron microscope (STEM) using a micrometer-sized liquid enclosure with electron transparent windows providing a wet specimen environment. Wet...

The aim of this study was to evaluate different methods for Trichomonas vaginalis diagnosis during pregnancy in order to prevent maternal and perinatal complications. A total of 386 vaginal exudates from pregnant women were analyzed. T. vaginalis was investigated by 3 types of microscopic examinations direct wet mount with physiologic saline solution, prolonged May-Grunwald Giemsa (MGG) stainin...

The ongoing Ebola virus (also known as Zaire ebolavirus, a member of the Ebolavirus family) outbreak in West Africa has so far resulted in >28000 confirmed cases compared with previous Ebolavirus outbreaks that affected a maximum of a few hundred individuals. Hence, Ebolaviruses impose a much greater threat than we may have expected (or hoped). An improved understanding of the virus biology is ...

Vertical hydraulic gradient is commonly measured in rivers, lakes, and streams for studies of groundwater-surface water interaction. While a number of methods with subtle differences have been applied, these methods can generally be separated into two categories; measuring surface water elevation and pressure in the subsurface separately or making direct measurements of the head difference with...

In this chapter, we describe a few popular experimental methodologies that can be used to independently validate the results of a microarray experiment. In contrast to many of the other chapters in this book, the techniques described here are decidedly non-mathematical or statistical in nature. Instead, most of them utilize standard 'wet-bench' molecular biology protocols that have proven thems...

A life cycle assessment of the impact of distillers grains plus solubles (DGS) on mitigation of energy and greenhouse gas (GHG) emissions comparing corn ethanol to gasoline demonstrates the importance of feeding wet DGS (WDGS) to feedlot cattle to optimize the environmental benefit of ethanol production relative to gasoline. Ethanol produced in Nebraska has a superior environmental impact compa...

A hollow cylinder apparatus was used to study some engineering properties of hollow cylindrical sand specimens prepared using the wet-pluviation technique. The refined specimen preparation method displayed good repeatability. Wet pluviation produced inherently cross-anisotropic specimens, with the level of initial anisotropy reducing with decreasing initial void ratio. The variation in the init...