To transfer only part of genome, X-ray irradiated suspension cell protoplasts of N. Ian gsdorfii were fused with suspension cell protoplasts of N. glauca by polyethylene glycol. Somatic hybrid calli were selected by the growth in the hormone-free medium. Some of somatic hybrid calli from fusion with irradiated protoplasts indicated the loss of small subunit polypeptide of fraction 1 protein whi...

background: the rise of opportunistic fungal infections highlights the need for development of new antimicrobial agents. antimicrobial peptides (amps) and antifungal peptides (afps) are among the agents with minimal resistance being developed against them, therefore they can be used as structural templates for design of new antimicrobial agents. methods: in the present study four antifungal pep...

A simple procedure is described for the mechanical isolation of protoplasts of unfertilized and fertilized barley egg cells from dissected ovules. Viable protoplasts were isolated from ~75% of the dissected ovules. Unfertilized protoplasts did not divide, whereas almost all fertilized protoplasts developed into microcalli. These degenerated when grown in medium only. When cocultivated with barl...

Blue light was found to induce shrinkage of the protoplasts isolated from first-leaf lamina pulvini of 18-day-old Phaseolus vulgaris. The response was transient following pulse stimulation, while it was sustainable during continuous stimulation. No apparent difference was found between flexor and extensor protoplasts. Protoplasts of the petiolar segment located close to the pulvinus showed no d...

A preparation of pure protoplasts of Geotrichum candidum became osmotically stable and colonies developed when the protoplasts were embedded in stabilizing thin-layer-agar and incubated with stabilizing basal medium. When growing protoplasts were exposed to distilled water and then reincubated with basal medium, the process of regeneration of protoplasts could be quantitatively demonstrated by ...

Protoplasts of Bacillus megaterium grew well and divided in nutrient broth containing 0.5 m NaCl as the stabilizer. Protoplasts also grew when sucrose or succinate was used instead of NaCl; however, no division phenomena were observed. The sequence of growth and division was similar to the results obtained by McQuillen. Protoplasts enlarged from 1.8 to 3.5 mu in diameter, and then a small protu...

A method for determining the subcellular metabolite levels in spinach protoplasts is described. The protoplasts are disrupted by centrifugation through a nylon net, releasing intact chloroplasts which pass through a layer of silicone oil into perchloric acid while the remaining cytoplasmic components remain over the oil and are simultaneously quenched as acid is centrifuged into them. Cross-con...

Sieve element (SE) protoplasts were liberated by exposing excised phloem strands of Vicia faba to cell wall-degrading enzyme mixtures. Two types of SE protoplasts were found: simple protoplasts with forisome inclusions and composite twin protoplasts-two protoplasts intermitted by a sieve plate-of which one protoplast often includes a forisome. Forisomes are giant protein inclusions of SEs in Fa...

Phenotypical alterations observed in rolB-transformed plants have been proposed to result from a rise in intracellular free auxin due to a RolB-catalyzed hydrolysis of auxin conjugates (J.J. Estruch, J. Schell, A. Spena [1991] EMBO J 10: 3125-3128). We have investigated this hypothesis in detail using tobacco (Nicofiana tabacum) mesophyll protoplasts isolated from plants transformed with the ro...

Many streptomycetes strains are hardly or not at all transformable via protoplasts, there is a problem with the regeneration of protoplasts. We found that protoplasts formed directly in cultivation media under submerged conditions presence lytic enzyme. Actinophage ?1/6 endolysin and lysozyme were used this study. Streptomyces cultivated several glycine enzyme for 24 48h. The highest amounts (a...