We have studied semithin frozen sections of developing and adult rat central nervous system (CNS) by indirect immunofluorescence in order to determine the antigenic phenotype of protoplasmic and fibrous astrocytes. Using antibodies against glial fibrillary acidic protein (GFAP) to identify astrocytes, we show that the great majority of fibrous astrocytes in adult optic nerve are labeled by the ...

We construct electronic oscillator from acellular slime mould Physarum polycephalum. The slime mould oscillator is made of two electrodes connected by a protoplasmic tube of the living slime mould. A protoplasmic tube has an average resistance of 3 MOhm. The tube’s resistance is changing over time due to peristaltic contractile activity of the tube. The resistance of the protoplasmic tube oscil...

It has been shown (Weibull, 1953) that it is possible to isolate protoplasts from Bacillus megaterium by lysozyme treatment of cells suspended in 0.1 to 0.2 M sucrose. When protoplasts are resuspended in phosphate buffer (pH 7), rapid lysis occurs, and the protoplasmic constituents are released. This procedure does not include any mechanical disintegration; in addition, only a small fraction of...

The distribution and rates of exchange of the ions sodium, potassium, and chloride in single internodal cells of the ecorticate characean, Nitdlopsis obtusa, have been studied. In tracer experiments three kinetic compartments were found, the outermost "free space" of the cell, a compartment we have called "protoplasmic non4ree space", and the cell sap. The concentrations in the vacuole were 54 ...

In electron microscopic studies, protoplasmic anastomoses were found to occur between smooth muscle cells in the gastrointestinal tracts of rats. The protoplasmic connections are apparently cylindrical in form and contain cytoplasm having a density similar to that of the cellular regions connected. The membranes enclosing the cytoplasmic connections are continuous with the membranes of the conn...

When cultures of Brachyspira hyodysenteriae were grown under a wide range of in vitro conditions, at least 1% of the cells formed spherical bodies different to the normal helical form. This percentage increased considerably in aging cultures or following their incubation in caramelized media. Spherical body formation was initiated from a terminal localized swelling of the outer sheath followed ...

The distribution and rates of exchange of the ions sodium, potassium, and chloride in single internodal cells of the ecorticate characean, Nitellopsis obtusa, have been studied. In tracer experiments three kinetic compartments were found, the outermost "free space" of the cell, a compartment we have called "protoplasmic non-free space", and the cell sap. The concentrations in the vacuole were 5...