Background: Despite advances toward an improved understanding of the evasive mechanisms leading to the establishment of cystic echinococcosis, the discovery of specific immunosuppressive mechanisms and related factors are of great interest in the development of an immunotherapeutic approach. Objective: To elucidate immunosuppressive effects of bioactive factors contained in chromatographic frac...

The objective of this study was to identify and evaluate for the first time anticoagulant fibrinogenolytic protease from Psidium guajava seed extract. extract in sodium dodecyl sulfate polyacrylamide gel electrophoresis showed protein bands high molecular weight region between 122 kDa 47 two distinct lower at ~16.6 ~14.3 kDa. content found be 1 mg/ml with specific activity 0.8 U. proteolytic su...

Proteolytic bacteria are most important for industries such as food and fermentation. Thus isolating and manipulating pure culture from various sources has importance for various biotechnology industries. In the present investigation a total of 26 bacteria were isolated from the dairy plant effluent. All isolates were screened for proteolytic activity using alkaline skim milk agar plate, among ...

A comparative study of the proteolytic enzymes production using twelve Aspergillus strains previously unused for this purpose was performed by solid state fermentation. A semiquantitative and quantitative evaluation of proteolytic activity were carried out using crude enzymatic extracts obtained from the fermentation cultures, finding seven strains with high and intermediate level of protease a...

The current study explores the anticoagulant, fibrinogenolytic and anti-platelet aggregation activities of Lablab purpureus (L.) Sweet seed radicle extract (LPRE). It is firstly reported that LPRE has protein at a concentration 1.5 mg/ml further evaluated for in gel electrophoresis. proteolytic activity was using casein gelatin as substrate. showed specific 0.35 U. increased plasma clotting tim...

Cell extracts from Pyrococcus furiosus were found to contain five proteases, two of which (S66 and S102) are resistant to sodium dodecyl sulfate (SDS) denaturation. Cell extracts incubated at 98 degrees C in the presence of 1% SDS for 24 h exhibited substantial cellular proteolysis such that only four proteins could be visualized by amido black-Coomassie brilliant blue staining of SDS-polyacryl...

The aim of this study was to investigate the effect of substitution of NaCl with KCl at different pH levels and salt concentrations on proteinase activity of cell-free extract and cell-free supernatant of the probiotics Lactobacillus acidophilus and Lactobacillus casei. de Man, Rogosa, and Sharpe broth aliquots were mixed with 2 pure salts (NaCl and KCl) and 2 salt concentrations at 2 concentra...

Several species of the family Bromeliaceae are characterized by the production of proteases in unusual amounts, especially in fruits. Bromelain, an extract rich in cysteine endopeptidases obtained from Ananas comosus L., and a few other proteases have been used as anti-inflammatory agents for some years, but bromelain is still mainly being used as alternative and/or complementary therapy to the...

There is an increased interest in obtaining amaranth grain extracts enriched with biologically active substances by deep processing of raw materials a further prospect their use as part specialized multi-purpose food products. The aim this work was to evaluate the effectiveness membrane filtration for extraction from grains. Amaranth extract obtained enzymatic hydrolysis using proteolytic enzym...

DNA-binding proteins that interact with the 3' end of the mouse mu immunoglobulin heavy chain gene were identified by the electrophoretic mobility shift assay. Complexes of distinctly different mobilities were formed by extracts prepared from B lymphoid lines representing different stages of maturation. The apparent stage-specific differences are shown to be due to proteolytic events that occur...