background: rabies virus (rabv) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. l protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication.objectives: a truncated l protein of rabies virus is being cloned, expressed and purified to produce relevant ...

the genome of iranian wheat stripe virus (iwsv), a tentative member of the genus tenuivirus, is comprised of three ambisense and one negative sense rna segments. the coat and non-structural proteins encoded by the vcrna3 and vrna4 genes, respectively, were efficiently translated in vitro. translated proteins of vcrna3 and vrna4 transcripts were approximately 35000 and 22000 in mr, respectively,...

brucellosis, caused by brucella spp., is an important zoonotic disease that causes abortion and infertility in cattle and undulant fever in humans. various studies have examined cell-free native and recombinant proteins as candidate protective antigens in animal models. among brucella immunogenes, antigen based on ribosomal preparation has been widely investigated. in this study, the immunogeni...

objective(s): streptokinase (ska) is an antigenic protein which is secreted by streptococcus pyogenes. streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. in the present study, recombinant streptokinase from s. pyogenes was produced and showed that recombinant ska protein was recognized by infected human sera using western blot analysi...

background bovine viral diarrhea (bvd) is an economically important disease of cattle with a worldwide distribution. diagnosis of bvd relies on laboratory-based detections of its viral causing agent or virus specific antibodies. the most common laboratory method used for this purpose is the elisa. bovine viral diarrhea virus (bvdv) nonstructural protein 3 (ns3) is one of the most highly conserv...

conclusions although the data support the idea that the basic mechanism of imipenem resistance could be via the loss of oprd, they do not fully explain the role of oprd and indicate that other mechanisms may play an important role. additionally, the significant decrease in the oprd levels in mdr strains suggests that oprd also plays a role in the emergence of both carbapenem and non-carbapenem ...

conclusions the gp40/15 gene, which was cloned in the pet28a+, was successfully expressed and produced in e. coli. therefore, this protein can be used in future studies to develop recombinant vaccines and diagnostic kits. methods in this experimental study, the gp40/15 gene sequence was extracted from genbank (no. af155624) and cloned in the pet28a+ plasmid. colony polymerase chain reaction (pc...

the development of a therapeutic vaccine against human papillomavirus (hpv) is important for the control of cervical cancer. e7 is the major transforming protein produced in cervical cancers, and therefore represents potential tumor-specific antigen that could be the target of immunotherapy for cervical cancer. among different vaccine strategies, protein-based vaccines are capable of generating...

the human papillomavirus l1 major capsid protein (hpv l1), the basis of the current vaccines, self-assembles into virus-like particles (vlps). herein, we describe the expression and purification of recombinant hpv16 l1 in e. coli system. the l1 protein was generated in a fused form using an inducible expression system. the recombinant gst-l1 fusion protein migrated as a 82 kda protein in sds-pa...

bacterial expression systems provide economic and logistic advantages in manufacturing proteins for human therapeutic purposes. however, most such proteins accumulate in insoluble biologicaiiy inactive form when overexpressed in bacterial cells. this is the case while attempting to produce recombinant human granulocyte-macrophage colony stimulating factor (hgm-csf) in e. coli. in this report, d...