background: one of the most important producers of high quality industrial enzymes is the gram-positive bacterium, bacillus subtilis (b. subtilis). one major limitation that hinders the wide application of b. subtilis is the secretion of high levels of extracellular proteases which degrade the secreted foreign proteins. in this study, homologus recombination technique was used to knock out its ...

Nowadays, textile processing based on biotechnology has gained its importance in the view of stringent environmental and industrial safety conditions. The use of protease enzymes in protein fibers to improve some physical and mechanical properties is particularly interesting. In this research, wool yarns were first treated with different concentrations of protease enzymes in aqueous solution in...

the aim of this study was to clone the serine alkaline protease-encoding gene from bacillus subtilis 168. this protease, which can have many applications especially in detergent, may be industrially an important enzyme. for the amplification of the gene, pcr was performed with a pair of primers specifically designed for this purpose. electrophoresis of the pcr product showed the expected band o...

Proteases are one of the predominant groups industrial enzymes and it represents for about 65% total global enzyme market. microbial origin have great importance over plant sources because they minimize production costs, increase characteristics desired products widely used in biotechnological process. Among protease enzymes, aspartic proteases most important proteolytic which mainly produced b...

Two 2 × 2 factorial experiments were conducted to investigate the interaction between xylanase (0 vs. 200 mg/kg) and protease (0 vs. 200 mg/kg) enzyme supplementation on growth performance (Exp. 1) and coefficient of ileal and total tract apparent digestibility in grower-finisher pigs (Exp. 2). One hundred and twenty-eight individual fed pigs (BW = 34.2 kg; n = 32) were assigned to 1 of 4 dieta...

The prokaryotic residents of the Tis solar saltern in the southeast of Iran on the shore of Oman Sea were investigated by the culture-dependent methods. Sequencing of the PCR-amplified fragments of 16S rRNA genes revealed that bacterial populations were related to Actinobacteria, Bacteroidetes, Balneolaeota, Firmicutes, and Proteobacteria. T...

BACKGROUND Fasciola hepatica is one of the most important helminthes parasites and triclabendazole (TCBZ) is routinely used for treatment of infected people and animals. Secreted protease enzymes by the F. hepatica plays a critical role in the invasion, migration, nutrition and the survival of parasite and are key targets for novel drugs and vaccines. The aim of study was to determine the prote...

The goal of this study was to isolate and identify Staphylococcus aureus from clinical environmental sources. This also looked at the prevalence distribution different virulence enzymes among its isolates. For objective, 65 samples were collected dispersed 50 distinct 15 (soil, water, air) samples. results revealed that 20 bacterial isolates S. aureus. isolation rate 30.76% all samples, with 13...

An intracellular serine protease, which is believed to initiate the degradation of several intracellular pyridoxal phosphate-dependent enzymes, was localized by immunofluorescence in atypical mast cells of the lamina propria and in intraepithelial cells of the rat small intestine. Some mucus-secreting goblet cells also contained the protease antigen. Atypical mast cells containing the enzyme we...