Background: Prolactin, a neurohormone, can act as a stimulator of immunity in a number of autoimmune diseases and its high levels have been shown in these diseases. Objective: This study was designed to discover the possible role of prolactin in autoimmune blistering skin diseases. The main goal was to compare the serum prolactin levels and the frequency of hyperprolactinemia in autoimmune skin...

DNA complementary to prolactin-specific sequences was prepared using a method which did not require purification of the prolactin mRNA. cDNA was synthesized using total cytoplasmic mRNA from a prolactinproducing rat pituitary cell line (GH,) as a template for avian myeloblastosis virus reverse transcriptase. A cDNA species 840 nucleotides in length was purified by electrophoresis on formamide p...

background premature rupture of membranes (prom) is one of the most common complications of pregnancy that has a major impact on pregnancy outcomes. a diagnostic tool that is non-invasive, specific and quick is needed to predict prom. the purpose of this study was to evaluate the diagnostic power of the vaginal washing fluid prolactin for the diagnosis of premature rupture of membranes and to d...

GH4C1 cells (GH cells) are a clonal strain of rat pituitary tumor cells which secrete prolactin. GH cells have been used to study hormone secretion, but they store relatively little prolactin compared to normal prolactin-secreting cells. They are not suitable, therefore, for studying some aspects of pituitary function. We have found that the amount of prolactin GH cells store can be regulated. ...

Establishment and maintenance of pregnancy require the activity of a highly specialized maternal tissue, the decidua. It is well established that the human decidua synthesizes and releases prolactin. However, in the rat, no study has been able to demonstrate the production of prolactin by the decidua. In this report, we established for the first time using Northern blot analysis and reverse tra...

In mammary epithelial cells (MECs), prolactin-induced signaling and gene expression requires integrin-mediated cell adhesion to basement membrane (BM). In the absence of proper cell-BM interactions, for example, culturing cells on collagen-coated plastic dishes, signal propagation is substantially impaired. Here we demonstrate that the RhoA-Rok-myosin II pathway accounts for the ineffectiveness...

Background: This study investigated the effects of estradiol on plasma calcium and prolactin cells of Heteropneustes fossilis kept in calcium-deficient and normal freshwater. Methods: Fish were deprived of food and divided into groups A-D. Group A and B were kept in artificial freshwater with normal electrolytes. Group C and D were maintained in calcium-deficient freshwater. Vehicle was admin...

Prolactin iodinated by lactoperoxidase method showed immunologically, electrophoretically and biolo9gically similar properties to native prolactin and possessed enough specific radioactivity for receptor studies. 1251-prolactin was incubated with mouse mammary tissues at 8 days of lactation. Both binding and release of 1251-prolactin depended on incubation time and temperature and were maximal ...

14 Since anterior pituitary expresses prolactin receptors, prolactin secreted by lactotropes could 15 exert autocrine or paracrine actions on anterior pituitary cells. In fact, it has been observed that 16 prolactin inhibits its own expression by lactotropes. Our hypothesis is that prolactin participates 17 in the control of anterior pituitary cell turnover. In the present study we explored the...

a total of 125 russian red pied cows were genotyped for the prolactim-related gene. the prl-rsai genotypes were analysed using the polymerase chain reaction -restriction fragment length polymorphism (pcr-rflp) method. in this breed, the frequencies of alleles were as follows;  a= 0.794 and b= 0.206. the frequencies of aa, ab and bb genotypes were 0.598, 0.392 and 0.01; respectively. results sho...