Plum pox virus (PPV) is a member of the Potyvirus genus of plant viruses. Labeling with UDP-[3H]galactose and galactosyltransferase indicated that the capsid protein (CP) of PPV is a glycoprotein with N-acetylglucosamine terminal residues. Mass spectrometry analysis of different PPV isolates and mutants revealed O-linked N-acetylglucosamination, a modification barely studied in plant proteins, ...

Plum pox virus (PPV) is the most damaging viral pathogen of stone fruits. The detection and identification of its strains are therefore of critical importance to plant quarantine and certification programs. Existing methods to screen strains of PPV suffer from significant limitations such as the simultaneous detection and genotyping of several strains of PPV in samples infected with different i...

Different mutants of an infectious full-length clone (p35PPV-NAT) of Plum pox virus (PPV) were constructed: three mutants with mutations of the assembly motifs RQ and DF in the coat protein gene (CP) and two CP chimeras with exchanges in the CP core region of Zucchini yellow mosaic virus and Potato virus Y. The assembly mutants were restricted to single infected cells, whereas the PPV chimeras ...

Sharka is a disease of fruiting, ornamental and wild Prunus species of great socio-economic relevance that has spread to most Prunusgrowing nations. It is caused by Plum pox virus (PPV), a member of the genus Potyvirus. In recent years, there have been notable advances in our understanding of genome organization and expression, functions of gene products, and pathogenicity and host range determ...

In comparison to single infections, co-infection of Nicotiana benthamiana with Potato virus X (PVX) and Potato virus Y (PVY) or Plum pox virus (PPV), resulted in increased systemic symptoms (synergism in pathology). Previous studies have shown that virus infections affected the accumulation of various microRNAs (miRNAs) and miRNA target genes. Our studies revealed that double infection by PVX a...

KAMENOVA, I., 2016. Non-transmission of plum pox virus through seeds of myrobalan and apricot. Bulg. J. Agric. Sci., 22: 267–271 Seeds from wild-grown myrobalan (Prunus cerasifera Ehrh.) infected with PPV-D strain and from apricot (P. armeniaca) infected with PPV-Rec strain were investigated for their potential role in virus transmission. The presence of PPV was checked in fully ripe seeds, gem...

The Plum pox virus (PPV), is considered to be particularly serious in stone species, has appeared research and documents since 1915 from which revelations emerge about the first symptoms appearing orchards central eastern Europe, where (PPV) being so viral, it caused production losses species such as plum, nectarine, cherry, almond, sour nectarine last but not least apricot, were 100%. creation...

Abstract Plum pox virus (PPV) a potyvirus in the family Potyviridae , is etiologic agent of sharka, worldwide most important and detrimental viral disease stone fruits (genus Prunus ). Although PPV was identified Italy already 1970s it has been largely reported almost all Italian regions, broad investigation genetic characterization isolates lacking them, including Tuscany (Central Italy). To a...

Molecular techniques based on the polymerase chain reaction (PCR) can provide rapid and sensitive diagnosis of plum pox virus (PPV), the causal agent of the devastating 'sharka' disease of stone fruit trees. The present study compared routine polymerase chain reaction (PCR) procedures against a new system, PCR-ELISA (Boehringer Mannheim), which enables immunoenzymatic detection of PCR products....