We optimized proton-induced X-ray emission (PIXE) for tissue analysis in a toxicity-disposition study. We used cultured rabbit renal slices as the biological system to demonstrate the use of PIXE analysis. The renal slices were exposed to HgCl2, CdCl2, K2Cr2O7, or NaAsO2 alone or in a mixture. The PIXE analysis provides information on concentrations of elements above atomic number 11, and it is...

abstract heavy metals enter to human food chain through the plant and soil contamination. it is very important to evaluate the affecting factors on plant uptake of heavy metals. in this study the effects of copper and zinc availability on cadmium uptake of root and shoot of corn and sunflower was investigated. the experiment was carried out with a factorial arrangement, two species of plants (s...

1. Isolated surviving frog skin, when bathed with the same kind of diluted Ringer's solution on both sides, shows a negative correlation between net active salt uptake by the epithelium and spontaneous skin potential. Average values of 0.15 to 0.86 microeq. x hr.(-1) x cm.(-2) were measured and correlated with average skin potentials ranging from 107 to 25 mv. 2. Sodium uptake exceeded chloride...

this investigation aimed to examine the protective function of vitamin e on rat bone marrow mesenchymal stem cells (mscs) treated with cadmium chloride. rat bone marrow mscs were extracted using flashing-out and cultured in dmem containing 10% fbs and 100 u/ml pen/strep. at the end of the third passage, cells were divided into 4 groups including control, cadmium chloride, cadmium chloride + vit...

To evaluate the possibility that an apical membrane conductive pathway for oxalate is present in the rabbit distal ileum, we studied oxalate ([14C]oxalate) and chloride (36Cl) uptake into brush-border membrane vesicles enriched 15- to 18-fold in sucrase activity. Voltage-sensitive pathways for oxalate and chloride were identified by the stimulation of uptake provided by an inwardly directed pot...

In three separate sets of studies, the effects of ureteral ligation and coadministration of cadmium with cysteine or glutathione (GSH) (in either a 4:1 or 2:1 ratio of thiol to cadmium) on the renal disposition of cadmium were assessed in rats 1 h after the administration of cadmium. In all experiments, co-administration of cadmium with either cysteine or GSH caused the renal accumulation of ca...

objective(s): bone marrow mesenchymal stem cells (mscs) play an important role in bone health. cadmium causes osteoporosis, but the exact mechanisms of its effect on mscs are not known. materials and methods: rats were treated with cadmium chloride (40 mg/l) in drinking water for six weeks, and then the biochemical and morphological studies on mscs were carried out as a cellular backup for oste...

cadmium is a heavy metal with a strong adverse effect on crop quality. moreover, it is a mobile element in environment and plants can easily uptake and transfer it to their organs. cadmium can easily enter human and animals food chains and threatens their health. to study the effects of a water soluble polymer and inoculation with mycorrhizal fungi on cadmium uptake of clover grown on contamina...

1. Uptake of l-glutamate by Halobacterium salinarium is dependent on high concentrations of sodium chloride in the environment. When the sodium chloride is replaced by isomolar concentrations of potassium chloride, sodium acetate or potassium acetate, only negligible uptake occurs. 2. Most of the glutamate taken up can be shown to be in the cells in the free state and at a concentration of at l...

Sumarry. In wheat seedlings (Triticum durum), uptake of potassium and nitrate, transpiration and shoot growth were inhibited by adding cadmium to the nutrient medium. A sharp reduction in cytokinin content was observed within two hours of supplying cadmium. The reduction in cytokinin content was probably a result of hormone breakdown. In support of this, Cd-treatment elevated cytokinin oxidase ...