Background: Sperm cryopreservation has been associated with over production of reactive oxygen species (ROS). Buffalo spermatozoa are susceptible to ROS inducing damages due to insufficient level of cytoplsmic antioxidant along with high amount of poly unsaturated fatty acids composition in membrane structure. Therefore, appropriate antioxidant in buffalo cryopreservation extender would reduce ...

The objective was to determine the relationship between thawing time and post-thaw temperature when the final temperature of the semen was brought to 40 C. One ejaculate from each of 12 dairy bulls was packaged in .5-ml French straws. Semen in five straws from each ejaculate was thawed in a 35 c water bath for 12 s or 1 min. The straws were then immediately placed in water baths at 0, 10 or 20 ...

Ovulation time of gilts was controlled by oral treatment with altrenogest (17-allyl-hydroxyestra-4,9,11-trien-3-one) followed by gonadotropic hormone injections. Gilts were laparotomized 42 to 44 h after injection of human chorionic gonadotropin. Spermatozoa were surgically placed into ligated oviducts of gilts after one of the following semen treatments: frozen and thawed in Beltsville thawing...

The main objective of the current study was to explore the different activation mechanisms of capacitation and freeze-thawed spermatozoa. Using SDS-PAGE and Western blotting, the conversion process of boar proacrosin during freeze-thawing and capacitation of spermatozoa was analyzed. The results revealed that capacitated spermatozoa exhibited a greater fluorescence area than that of the freeze-...

The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and r...

The present study was performed to assess the fertility of frozen-thawed dog semen prepared by freezing with 6% glycerol and thawing at 70 degrees C for 8 sec, and to evaluate the least number of post-thaw spermatozoa necessary to achieve pregnancy by intrauterine or intratubal artificial insemination. It was found that the pregnancy rate of intrauterine artificial insemination was 100% using 6...

The aim of this study was to assess two models of sperm collection on the quality and fertility of thawed spermatozoa from Black Manchega rams, a threatened breed. Sperm samples were collected by electroejaculation and postmortem from each male. Samples were diluted with Biladyl and frozen. Motility (subjective and objective by means of computer-assisted semen analysis), membrane integrity, and...

BACKGROUND Sperm cryopreservation is the most effective method to preserve male fertility but this is normally used for motile spermatozoa. Thus, only motile spermatozoa are used for cryopreservation in most reproductive medicine centers worldwide. The immotile spermatozoa from some problematic patients are usually discarded, resulting in a missed opportunity of sterility cryopreservation for f...

Mammalian spermatozoa are extremely susceptible to high doses of reactive oxygen species (ROS). The aim of the present study was to investigate the potential role of glutamine in protecting rabbit spermatozoa against ROS stress during cryopreservation and post-thaw incubation. Freshly ejaculated semen was diluted with Tris-citrate-glucose extender supplemented with glutamine. The addition of 20...

OBJECTIVE To study the effect of freeze-thaw on embryos derived from intracytoplasmic sperm injection (ICSI) using surgically retrieved and ejaculated spermatozoa. DESIGN Retrospective study. SETTING Private IVF center. PATIENT(S) Three hundred eighty-three patients undergoing frozen-thawed ET cycles. INTERVENTION(S) Testicular sperm aspiration (TESA) or percutaneous epididymal sperm as...