Purification and PCR amplification procedures for DNA extracted from environmental samples (soil, compost, and river sediment) were improved by introducing three modifications: precipitation of DNA with 5% polyethylene glycol 8000 (PEG) and 0.6 M NaCl; filtration with a Sepharose 4B–polyvinylpolypyrrolidone (PVPP) spin column; and addition of skim milk (0.3% w/v) to the PCR reaction solution. H...

Premise The objective of this study was to optimize an existing DNA extraction protocol for recalcitrant plant taxa obtain high-quality from preserved herbarium tissue suitable downstream PCR applications. Methods and Results Leaf 30 diverse species obtained the U.S. National Arboretum Herbarium. Our previous (Gouker et al., 2020, Applications in Plant Sciences 8: e11403) improved by use 10X Tr...

We report an efficient, simple and cost-effective protocol for the isolation of genomic DNA from Ornithogalum species. Our modification of the standard CTAB protocol includes two polyphenol adsorbents (insoluble PVPP and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and addition of phenol to remove proteins and other contaminants. DNA yield obtained with our ...

The influence of the technological processes grape processing on content hydroxycinnamic acids and volatile phenolic substances in wine was studied. method targeted oxygenation applied technology Welschriesling Chardonnay varieties. determined by gas chromatography, liquid basic analytical parameters FTIR spectrometry. must had a statistically significant effect phenolics wine. In all three mon...

A highly efficient method for the oxidation of aldehydes to carboxylic acids using PolyVinylPolyPyrrolidone supported - Boron Trifluoride (PVPP-BF3) in the presence of 35% hydrogen peroxide has been developed in acetonitrile at 60 °C. This procedure cleanly oxidizes variety of aldehydes to the corresponding carboxylic acids. Oxidative esterification of benzaldeh...

Roesleria subterranea causes root rot in grapevine and fruit trees. The fungus has long been underestimated as a weak parasite, but during the last years it has been reported to cause severe damages in German vineyards. Direct, observation-based detection of the parasite is time consuming and destructive, as large parts of the rootstocks have to be uprooted and screened for the tiny, stipitate,...

Clopidogrel bisulphate (anti-coagulant drug) is an enantio-selective chrial drug and needs to be monitored for the active form in tablet formulation. Hence, a stable tablet formulation was developed for clopidogrel using two different disintegrants of LHPC (to match as closely as possible to innovator tablet) and PVPP XL. Tablets were evaluated for stability analysis using validated analytical ...

Abtracts. Two experiments were conducted to examine the effects of fining agents applied prior to and/or during fermentation on the color of Seyval blanc wine. In experiment one, kieselsol (Ke) at 719 mg/L; polyvinylpolypyrrolidone (PVPP) at 719 mg/L; bentonite (Bn) at 960 mg/L; combinations of these levels of Ke, PVPP, and Bn; sulfur dioxide (SO2) at 100 mg/L; and a control were applied to the...

We developed a protocol which yields purified bacterial DNA from the soil bacterial community. The bacteria were first dispersed and separated from soil particles in the presence of polyvinylpolypyrrolidone, which removes humic acid contaminants by adsorption to this insoluble polymer. The soil bacteria were then collected by centrifugation and lysed by using a comprehensive protocol designed t...