Large scale gene mapping efforts in domestic animals have generated and mapped a large number of genetic markers that are useful for mapping quantitative trait and disease loci and for DNA diagnostic purposes such as parentage testing. Marker polymorphism is an important criterion for selecting genetic markers in planning experiment for mapping quantitative trait loci or for DNA diagnostic purp...

in this study, genetic diversity of 20 wheat genotypes was evaluated using 126 simple sequence repeats (ssr) alleles, covering all three wheat genomes. a total of 1557 allelic variants were detected for 126 ssr loci. the number of alleles per locus ranged from 4 to 19 and the allelic polymorphism information content (pic) varied from 0.66 (xgwm429) to 0.94 (xgwm212 and xgwm515). the high­est po...

In the present study, we evaluated polymorphism of myogenic factor 5, involved in growth and meat quality traits. Based on PCR-SSCP technology, a novel missense substitution SNP (single-nucleotide polymorphism) g.1142 A > G was identifi ed in the intron1 region of the MyF-5 gene, it causes an amino acid substitution (1142Glutamine/ Glycine1142). Allele frequencies, gene heterozygosity, eff ecti...

Eight novel microsatellite loci from the genome of Fenneropenaeus penicillatus Alcock were developed using the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Thirty (30) wild individuals were used to analyze the polymorphism of these eight microsatellite markers. The results show that the number of alleles per locus and the polymor...

Twenty-four start codon targeted (SCoT) markers were used to assess genetic diversity and population structure of indigenous, introduced and domesticated ramie (Boehmeria nivea L. Gaudich.). A total of 155 genotypes from five populations were investigated for SCoT polymorphism, which produced 136 amplicons with 87.5% polymorphism. Polymorphism information content and resolving power of the SCoT...

This study of Iranian families assessed the usefulness of carrier detection of phenylketonuria by variable number tandem-repeat (VNTR) polymorphism analysis. We studied 171 people (45 unrelated PKU subjects, and their parents and unaffected siblings). Of 342 chromosomes (131 non-PKU and 211 PKU), 5 VNTR alleles were identified. This VNTR system would yield a polymorphism information content of ...

To identify cultivars quickly and accurately, DNA fingerprinting of 10 broccoli varieties was performed by using simple sequence repeat (SSR) marker technology. Highly informative polymorphic SSR markers were screened rapeseed. Out the 93 pairs, 21 pairs selected found to have good polymorphism. Each pair generated 1 bands with an average 4.29. The polymorphism information content (PIC) 0.41 a ...

PREMISE OF THE STUDY Senna spectabilis var. excelsa (Fabaceae) is a South and Central American tree of great ecological importance and one of the most common species in several sites of seasonally dry forests. Our goal was to develop microsatellite markers to assess the genetic diversity and structure of this species. METHODS AND RESULTS We designed and assessed 53 loci obtained from a micros...