results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...

background: acute infectious diarrhea is one of the most important causes of morbidity and mortality worldwide. objectives: the objective of this study was to characterize antimicrobial resistant diarrheagenic escherichia coli strains isolated from diarrheic children in tehran, ir iran. patients and methods: in total, 550 stool samples from diarrheic pediatric patients, aged less than 60 months...

background: human papillomaviruses (hpvs) are the most common viruses which can be sexually transmitted. they can cause different malignancies in asymptomatic women. the association of hpvs with infertility among men and women is controversial. in the current study, the authors compared the frequency of hpvs in fertile and infertile women in the city of mashhad. materials and methods: in the pr...

results of 50 patients, 20 were men and 30 women with mean age of 61.1 ± 16.9 years ranged between 22 and 85 years. all samples of pterygia had positive results for adenoviruses dna with polymerase chain reaction, but none of the negative control groups displayed adenoviruses. the pterygium group and the control groups were β-globin positive. direct sequencing of pcr products confirmed adenovir...

background aggregatibacter actinomycetemcomitans and tannerella forsythensis are two major pathogens in destructive periodontal disease in humans. the detection of these bacteria is needed for diagnosis and management of the mentioned diseases. objectives we aimed to develop and compare improved multiplex conventional and sybr green real time pcr assays for a specific diagnosis of the organisms...

conclusions: vegetables are a new source of h. pylori and accurate washing of vegetables improves such contaminations. results: the results showed that 44 of 460 samples (9.56%) were positive for h. pylori using the culture method. the polymerase chain reaction technique showed that 50 of 460 samples (10.86%) were positive for h. pylori. un-washed leek, traditional salad, un-washed basil and un...

abstract background brucellosis is endemic in iran, and is seen in all age groups, including children. its diagnosis in childhood needs a high index of suspicion. the diagnostic methods currently in use need evaluation for analytical performance. objective the present study tries to evaluate the hematological indices, serologic markers of inflammation and symptoms in patients suspected of bruce...

background: human enteroviruses (evs) may have a role as a possible risk factor in the pathogenesis of mi. the aim of this study was to evaluate the presence of enterovirus genomic rna in peripheral blood samples of patients with acute myocardial infarction (mi). methods: we investigated the presence of enterovirus genomic rna in the peripheral blood of 115 patients with acute mi hospitalized i...

background: vasopressin type 2 receptor (v2r) plays an important role in the water reabsorption in the kidney collecting ducts. v2r is a g protein coupled receptor (gpcr) and the triplet of amino acids aspartate-arginine-histidine (drh) in this receptor might significantly influence its activity similar to other gpcr. however, the role of this motif has not been fully confirmed. therefore, the ...