نتایج جستجو برای: polymerase chain reactionn

تعداد نتایج: 352457  

Journal: :jundishapur journal of microbiology 0
parisa mohammadi department of microbiology, faculty of biological sciences, alzahra university, tehran, ir iran; department of microbiology, faculty of biological sciences, alzahra university, tehran, ir iran. tel: +98-2185692714, fax: +98-2188058912 aida hamidkhani department of biotechnology, faculty of biological sciences, alzahra university, tehran, ir iran ezat asgarani department of biotechnology, faculty of biological sciences, alzahra university, tehran, ir iran

results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...

Journal: :iranian red crescent medical journal 0
maryam heidary department of microbiology, falavarjan branch, islamic azad university, falavarjan, ir iran hassan momtaz department of microbiology, shahrekord branch, islamic azad university, shahrekord, ir iran; department of microbiology, shahrekord branch, islamic azad university, shahrekord, ir iran. tel/fax: +98-3361064 mahboobeh madani department of microbiology, falavarjan branch, islamic azad university, falavarjan, ir iran

background: acute infectious diarrhea is one of the most important causes of morbidity and mortality worldwide. objectives: the objective of this study was to characterize antimicrobial resistant diarrheagenic escherichia coli strains isolated from diarrheic children in tehran, ir iran. patients and methods: in total, 550 stool samples from diarrheic pediatric patients, aged less than 60 months...

Journal: :archives of medical laboratory sciences 0
aida javanbakht zahra meshkat nayereh khadem ghaebi mojtaba meshkat sedigheh ayati afin narges javan

background: human papillomaviruses (hpvs) are the most common viruses which can be sexually transmitted. they can cause different malignancies in asymptomatic women. the association of hpvs with infertility among men and women is controversial. in the current study, the authors compared the frequency of hpvs in fertile and infertile women in the city of mashhad. materials and methods: in the pr...

Journal: :jundishapur journal of microbiology 0
fatemeh sadat ghasemi department of parasitology, faculty of medicine, kashan university of medical sciences, kashan, ir iran sima rasti department of parasitology, faculty of medicine, kashan university of medical sciences, kashan, ir iran; department of parasitology, faculty of medicine, kashan university of medical sciences, kashan ir iran. tel: +98-9132611568, fax: +98-3615551112 mojgan bandehpour cellular and molecular biology research center, shahid beheshti university of medical sciences, tehran, ir iran; biotechnology department, faculty of medicine, shahid beheshti university of medical sciences, tehran, ir iran bahram kazemi cellular and molecular biology research center, shahid beheshti university of medical sciences, tehran, ir iran; biotechnology department, faculty of medicine, shahid beheshti university of medical sciences, tehran, ir iran ahmad piroozmand department of microbiology and immunology, faculty of medicine, kashan university of medical sciences, kashan, ir iran gholam abbas mousavi department of statistics and public health, faculty of health, kashan university of medical sciences, kashan, ir iran

Journal: :jundishapur journal of microbiology 0
mishar kelishadi infectious diseases research center, golestan university of medical sciences, gorgan, ir iran mandana kelishadi department of ophthalmology, 5th azar hospital, gorgan, ir iran abdolvahab moradi infectious diseases research center, golestan university of medical sciences, gorgan, ir iran naeme javid infectious diseases research center, golestan university of medical sciences, gorgan, ir iran masoud bazouri infectious diseases research center, golestan university of medical sciences, gorgan, ir iran alijan tabarraei infectious diseases research center, golestan university of medical sciences, gorgan, ir iran; infectious diseases research center, golestan university of medical sciences, gorgan, ir iran. tel: +98-1714422652, fax: +98-1714440225

results of 50 patients, 20 were men and 30 women with mean age of 61.1 ± 16.9 years ranged between 22 and 85 years. all samples of pterygia had positive results for adenoviruses dna with polymerase chain reaction, but none of the negative control groups displayed adenoviruses. the pterygium group and the control groups were β-globin positive. direct sequencing of pcr products confirmed adenovir...

Journal: :jundishapur journal of microbiology 0
mohammad soleimani department of microbiology, qom branch, islamic azad university, qom, ir iran; department of microbiology, qom branch, islamic azad university, qom, ir iran, p.o. box: 37185/364. tel:+98-2517780001, fax: +98-2517780001 mohammad reza zolfaghari department of microbiology, qom branch, islamic azad university, qom, ir iran abbas morovvati department of microbiology, qom branch, islamic azad university, qom, ir iran

background aggregatibacter actinomycetemcomitans and tannerella forsythensis are two major pathogens in destructive periodontal disease in humans. the detection of these bacteria is needed for diagnosis and management of the mentioned diseases. objectives we aimed to develop and compare improved multiplex conventional and sybr green real time pcr assays for a specific diagnosis of the organisms...

Journal: :jundishapur journal of microbiology 0
shahrzad atapoor faculty of agriculture and natural sciences, shahrekord branch, islamic azad university, shahrekord, ir iran farhad safarpoor dehkordi young researchers and elites club, shahrekord branch, islamic azad university, shahrekord, ir iran ebrahim rahimi department of food hygiene and public health, college of veterinary medicine, shahrekord branch, islamic azad university, shahrekord, ir iran; department of food hygiene and public health, college of veterinary medicine, shahrekord branch, islamic azad university, shahrekord, ir iran. tel: +98-9133278377, fax: +98-3813361060

conclusions: vegetables are a new source of h. pylori and accurate washing of vegetables improves such contaminations. results: the results showed that 44 of 460 samples (9.56%) were positive for h. pylori using the culture method. the polymerase chain reaction technique showed that 50 of 460 samples (10.86%) were positive for h. pylori. un-washed leek, traditional salad, un-washed basil and un...

Journal: :iranian journal of pediatric hematology and oncology 0
mb khalili associate professor of microbiology, dept. of microbiology, school of medicine, shahid sadoughi university of medical sc m sadeh phd student, msc of microbiology, dept. of laboratory sciences, school of paramedicine, shahid sadoughi university of meسازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید صدوقی یزد (shahid sadooghi university of medical sciences) s hekmati moghaddam assistant professor of pathology, dept. of laboratory sciences, school of paramedicine, shahid sadoughi university of meسازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید صدوقی یزد (shahid sadooghi university of medical sciences)

abstract background brucellosis is endemic in iran, and is seen in all age groups, including children. its diagnosis in childhood needs a high index of suspicion. the diagnostic methods currently in use need evaluation for analytical performance. objective the present study tries to evaluate the hematological indices, serologic markers of inflammation and symptoms in patients suspected of bruce...

Journal: :iranian journal of public health 0
t mohamadpoor ms nabavinia a gholoobi ms alavi z meshkat

background: human enteroviruses (evs) may have a role as a possible risk factor in the pathogenesis of mi. the aim of this study was to evaluate the presence of enterovirus genomic rna in peripheral blood samples of patients with acute myocardial infarction (mi). methods: we investigated the presence of enterovirus genomic rna in the peripheral blood of 115 patients with acute mi hospitalized i...

Journal: :iranian biomedical journal 0
حسین نجف زاده hossein najafzadeh لیلا صفائیان leila safaeian حمید میر محمد صادقی hamid mirmohammad sadeghi محمد ربانی mohammad rabbani عباس جعفریان abbas jafarian

background: vasopressin type 2 receptor (v2r) plays an important role in the water reabsorption in the kidney collecting ducts. v2r is a g protein coupled receptor (gpcr) and the triplet of amino acids aspartate-arginine-histidine (drh) in this receptor might significantly influence its activity similar to other gpcr. however, the role of this motif has not been fully confirmed. therefore, the ...

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