MATERIALS 5x TBE electrophoresis buffer Polyacrylamide gels are poured and run in 0.5x or 1x TBE at low voltage (1-8 V/cm) to prevent denaturation of small fragments of DNA by Joulic heating. Other electrophoresis buffers such as 1x TAE (please see Agarose Gel Electrophoresis) can be used, but they are not as good as TBE. The gel must be run more slowly in 1x TAE, which does not provide as much...

Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a freeradical polymerization that requires an initiator, usually ammonium persulfate, and a catalyst like N,N,N’,N’-tetraethylmethylenediamine (TEMED). A distinct advantage of polyacrylamide gel systems is that by ...

Background. Open wound treatment requires a use of bandage material to prevent the development pathogenic microflora and provide necessary conditions for tissue regeneration.
 The aim study was compare effectiveness polyacrylamide (PAA) dextran-graft-polyacrylamide (D-PAA) hydrogels loaded with silver nanoparticles (AgNPs), antibiotics, photosensitizers bacterial infection open wounds.&#x0...

chemical flooding, especially alkaline/surfactant/polymer flooding, is of increasing interest due to the world increasing oil demand. this work shows the aspects of using alkaline/surfactant/polymer as an enhanced oil recovery method in the porous media having a high dead-end pore frequency with various dead-end pore parameters (such as opening, depth, aspect ratio, and orientation). using glas...

a gel composite (based on polyacrylamide and crosslinker cr(iii) acetate) was prepared by solvent method (distillate and water formation) and nanoclay particle (kaolinite). using xrd tests, d001 was evaluated for kaolinite nanoparticles in gel composite. kaolinite modification using dimethyl-sulfoxide led to increase interlamellar spacing from 7.21 to 10.82 o a. based on the results obtained fo...

conclusions our method provided good results and performing evacuation under the guide of bedside ultrasonography is recommended. results we treated 173 patients, including 164 females (94.8%), with the mean (sd) age of 43.75 (10.62) years. according to vas, 95.4% of patients reported complete satisfaction while 2 (1.2%) were unsatisfied with the results. patients reported no complication of th...

drilling fluid is the most important lifeline of the drilling operation, that main task is facilitate the cuttings removal of the drilling. there are varieties of drilling fluids such as sodium bentonite based-drilling fluid is called “mud” and drilling foam or surfactant based-drilling fluid is called “soap”. the present work aims are study on the modified drilling mud properties by using the ...

introduction when a liquid is irradiated with high-intensity and low-frequency ultrasound, acoustic cavitation occurs and there are some methods to determine and quantify this phenomenon. the existing methods for performing these experiments include sonochemiluminescence (scl) and chemical dosimetric methods. the particles in a liquid decrease the ultrasonic intensity threshold needed for cavit...

Evaluation of Schiff base nanocomposite hydrogels properties using a benzaldehyde multifunctional amphiphilic polyacrylamide crosslinker in conjunction with glycol chitosan.

Geopolymers are a new type of environmentally friendly cement-based material with serious drying shrinkage problems. In order to overcome this problem and improve the engineering performance durability geopolymers, in study we added 0%, 0.3%, 0.5%, 0.7%, 0.9% polymer materials, namely, polyacrylamide, sodium polyacrylate, tetraborate, respectively, geopolymers reduce their degree shrinkage. We ...