In the current series of experiments we investigated the role of bradykinin in airway hyperresponsiveness induced by human eosinophil-granule major basic protein (MBP). Bronchoalveolar lavage was performed after intratracheal instillation of MBP or poly-L-lysine in anesthetized, intubated rats, and levels of immunoreactive kinins and kallikrein-like activity were determined. Both MBP and poly-L...

Figure S1: molecules used to chemically functionalize the surfaces. To the left, 3-Aminopropyl)-triethoxysilane (APTES). To the right, poly-L-lysine hydro bromide. Figure S2: Morphological AFM images. Scale bar is 10 µm A: Si/SiO 2 coated with 3-Aminopropyl)-triethoxysilane (APTES), B: Si/SiO 2 coated with, poly-L-lysine hydro bromide and C: Bare Si/SiO 2 .

We present, here, evidence that foreign DNA can be specifically delivered to cells by a soluble carrier system that takes advantage of receptor-mediated endocytosis. Our experiments were based on the following concepts: hepatocytes possess a unique receptor that binds and internalizes galactose-terminal (asialo-)glycoproteins; DNA can bind to polycations in a strong but noncovalent manner formi...

AIMS To evaluate two of the recent methods of coating microtitre plates in the enzyme linked immunosorbent assay (ELISA) for detecting human antibodies against meningococcal capsular polysaccharides A and C with a view to validating a specific meningococcal antibody assay for routine clinical use. METHODS Two four-layer ELISA protocols were standardised: one method utilised meningococcal poly...

Antisense oligonucleotides represent an interesting tool for selective inhibition of gene expression, but their efficient introduction within intact cells proved to be difficult to realize. As a step toward this goal, small (13- or 15-mer) synthetic oligodeoxyribonucleotides have been coupled at their 3' ends to epsilon-amino groups of lysine residues of poly(L-lysine) (Mr, 14,000). A 15-mer ol...

Efficient initiation from ammonium trifluoroacetate salts (TFA) dendritic end-groups yields well-defined poly( l -lysine) star polypeptides with 8, 16 and 32 arms. Hydrolytic core degradation plasmid DNA complexation is demonstrated.

Light-absorption spectra of complexes between reduced iron-protoporphyrin IX (2 X 10-S M) and various polylysine samples (up to 2 X 1OW residue molar) were measured in the range 380-630 rnp in deaerated aqueous solutions of pH 12 and at 27”. The absorption spectra obtained were very similar to well-known ferrohemochrome spectra of proteins or of complexes of protoheme with low-molecular-weight ...

Block copolymers comprising a poly(styrene) and a poly(L-lysine) or poly(L-glutamic acid) block were obtained by sequential reversible addition−fragmentation chain Transfer (RAFT) and N-carboxyanhydride (NCA) polymerization. Subsequent partial glycosylation of the poly(L-glutamic acid) block with D-galactosamine (GA) and the poly(L-lysine) block with lactobionic acid (LA) yielded block copolyme...