نتایج جستجو برای: pluripotency genes

تعداد نتایج: 430013  

Journal: :The EMBO journal 2012
A Aziz Aboobaker Damian Kao

Stem cells, both adult and germline, are the key cells underpinning animal evolution. Yet, surprisingly little is known about the evolution of their shared key feature: pluripotency. Now using genome-wide expression profiling of pluripotent planarian adult stem cells (pASCs), Önal et al (2012) present evidence for deep molecular conservation of pluripotency. They characterise the expression pro...

2016
Jing Hu Zhiyong Yang Jinbo Wang Jia Yu Jing Guo Shiying Liu Chunmei Qian Liwen Song Yi Wu Jiajing Cheng Lygia V. Pereira

An improved understanding of the pluripotency maintenance of embryonic stem (ES) cells is important for investigations of early embryo development and for cell replacement therapy, but the mechanism behind pluripotency is still incompletely understood. Recent findings show that zinc, an essential trace element in humans, is critically involved in regulating various signaling pathways and genes ...

Journal: :Biotechnology and bioengineering 2004
Raj R Rao John D Calhoun Xiaoting Qin Romdhane Rekaya Jason K Clark Steven L Stice

Human embryonic stem cells (ESCs) have generated enormous interest due to their ability to self-renew and produce many different cell types. In conjunction with microarray technology, human ESCs provide a powerful tool for employing a systems-based approach to deciphering the molecular mechanisms that control pluripotency and early development. Recent work has focused on defining "stemness" and...

2013
Hedi Peterson Raed Abu Dawud Abhishek Garg Ying Wang Jaak Vilo Ioannis Xenarios James Adjaye

Pluripotency in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) is regulated by three transcription factors-OCT3/4, SOX2, and NANOG. To fully exploit the therapeutic potential of these cells it is essential to have a good mechanistic understanding of the maintenance of self-renewal and pluripotency. In this study, we demonstrate a powerful systems biology approach ...

Journal: :Gene 2012
Anup Som Mitja Luštrek Nitesh Kumar Singh Georg Fuellen

Identification of the key genes/proteins of pluripotency and their interrelationships is an important step in understanding the induction and maintenance of pluripotency. Experimental approaches have accumulated large amounts of interaction/regulation data in mouse. We investigate how far such information can be transferred to human, the species of maximum interest, for which experimental data ...

2011
Thorold W. Theunissen Yael Costa Aliaksandra Radzisheuskaya Anouk L. van Oosten Fabrice Lavial Bertrand Pain L. Filipe C. Castro José C. R. Silva

Pluripotency is a developmental ground state that can be recreated by direct reprogramming. Establishment of pluripotency is crucially dependent on the homeodomain-containing transcription factor Nanog. Compared with other pluripotency-associated genes, however, Nanog shows relatively low sequence conservation. Here, we investigated whether Nanog orthologs have the capacity to orchestrate estab...

2014
Nishant Singhal Daniel Esch Martin Stehling Hans R. Schöler

BAF chromatin remodeling complexes containing the BRG1 protein have been shown to be not only essential for early embryonic development, but also paramount in enhancing the efficiency of reprogramming somatic cells to pluripotency mediated by four transcription factors. To investigate the role of BRG1 in regulating pluripotency, we found that Oct4 and Nanog levels were increased immediately aft...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2009
Jiaxi Zhou Pei Su Lu Wang Joanna Chen Maike Zimmermann Olga Genbacev Olubunmi Afonja Mary C Horne Tetsuya Tanaka Enkui Duan Susan J Fisher Jiayu Liao Jie Chen Fei Wang

Despite the recent identification of the transcriptional regulatory circuitry involving SOX2, NANOG, and OCT-4, the intracellular signaling networks that control pluripotency of human embryonic stem cells (hESCs) remain largely undefined. Here, we demonstrate an essential role for the serine/threonine protein kinase mammalian target of rapamycin (mTOR) in regulating hESC long-term undifferentia...

Journal: :Stem cells 2015
Galbha Duggal Sharat Warrier Sabitri Ghimire Dorien Broekaert Margot Van der Jeught Sylvie Lierman Tom Deroo Luc Peelman Ann Van Soom Ria Cornelissen Björn Menten Pieter Mestdagh Jo Vandesompele Matthias Roost Roderick C Slieker Bastiaan T Heijmans Dieter Deforce Petra De Sutter Susana Chuva De Sousa Lopes Björn Heindryckx

Human embryonic stem cells (hESCs) closely resemble mouse epiblast stem cells exhibiting primed pluripotency unlike mouse ESCs (mESCs), which acquire a naïve pluripotent state. Efforts have been made to trigger naïve pluripotency in hESCs for subsequent unbiased lineage-specific differentiation, a common conundrum faced by primed pluripotent hESCs due to heterogeneity in gene expression existin...

2016
Weiyu Zhang Peiling Ni Chunlin Mou Yanqin Zhang Hongchao Guo Tong Zhao Yuin-Han Loh Lingyi Chen

The COP9 signalosome has been implicated in pluripotency maintenance of human embryonic stem cells. Yet, the mechanism for the COP9 signalosome to regulate pluripotency remains elusive. Through knocking down individual COP9 subunits, we demonstrate that Cops2, but not the whole COP9 signalosome, is essential for pluripotency maintenance in mouse embryonic stem cells. Down-regulation of Cops2 le...

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