A direct relation between gene dosage and amount of the corresponding protein is generally assumed to exist though it has been investigated in a limited number of cases. Yeasts seem to be an ideal material for this type of investigation as they can be easily obtained in different degrees of ploidy. Macromolecular components of yeast cells in polyploid series have been already determined (OGUR e...

Okra Abelmoschus esculentus L. (Moench), is an economically important vegetable crop grown in tropical and sub-tropical as well as Mediterranean countries. The genome size of the Abelmoschus genus species is still rare. Ploidy determinations have traditionally been done by counting chromosomes of stained root tips, but this method is laborious and often difficult with species which have small c...

A method for the determination of the DNA content of isolated nuclei of different ploidy has been developed. It is based on measurement of the nuclear dry mass, with an integrating microinterferometer, before and after DNase treatment. The values found are slightly low, because, as indicated by biochemical determinations, consistently 5% to 8% of DNA is not extracted by DNase under these condit...

Transitional cell carcinoma(T.C.C) of bladder display an upredictable biological behavior.Morphologic methods of grading this tumor are insuficient to predict the clinical outcome of the patients.the aim of this study was to investigate the relationships between histological grading staging and DAN ploidy especially in intermediate grade II(IIa and IIb). in a retrospective study  of tissue spec...

Cytogenetic characterization and determination of DNA content by flow cytometry of five species of Mecardonia Ruiz et Pavon, 1798 (Gratiolae, Plantaginaceae) was performed. This is the first study of nuclear DNA content carried out in the genus. Mitotic analysis revealed a base chromosome number x = 11 for all entities and different ploidy levels, ranging from diploid (2n = 2x = 22) to hexaploi...

The ploidy distribution of megakaryocytes shifts in response to platelet demand and thus provides a sensitive index of megakaryocytopoiesis. Flow cytometry (FCM) is a potentially valuable method for rapid determination of ploidy distributions of megakaryocyte populations; however, because megakaryocytes constitute only a very small proportion of the cells in unfractionated marrow, other rare ev...

Males in many animal species differ greatly from females in morphology, physiology and behaviour. Ants, bees and wasps have a haplodiploid mechanism of sex determination whereby unfertilized eggs become males while fertilized eggs become females. However, many species also have a low frequency of diploid males, which are thought to develop from diploid eggs when individuals are homozygous at on...