The present study aimed to determine the antitumor effects of polysaccharides extracted from Pleurotus ostreatus mycelium on gastric cancer in vitro and in vivo. Polysaccharides were extracted from Pleurotus ostreatus mycelium and an antitumor component, known as Pleurotus ostreatus mycelium polysaccharides 2 (POMP2), with a relative molecular weight of 29 kDa, was then sequentially purified us...

We selected Pleurotus ostreatus from among several edible mushrooms because it has high anti-gout xanthine oxidase (XOD) inhibitory activity. The maximal amount of XOD inhibitor was extracted when the Pleurotus ostreatus fruiting body was treated with distilled water at 40℃ for 48 hr. The XOD inhibitor thus obtained was purified by Sephadex G-50 gel permeation chromatography, ultrafiltration, C...

Pleurotus ostreatus (MTCC142) was cultivated on three different agro wastes viz: Wheat straw, Soybean straw and Chick pea straw. Total antioxidant, reducing power and total phenolic content effect of the extracts was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and Folin-Ciocalteu method. The aqueous extract of P.ostreatus cultivated on soybean straw exhibited good antioxidant acti...

Carbon, hydrogen and nitrogen analyses of banana leaf and pseudostem biomass revealed their potentiality as substrates for microorganisms. Infra-red (IR) spectra of both biomass show presence of cellulose, xylan and lignin. IR spectra of leaf and pseudostem biomass degraded in solid state fermentation (SSF) by two Pleurotus species (P. sajor-caju and P. ostreatus) for 40 days showed the utiliza...

In this work, the production of fungal laccase was optimized from local isolate of Pleurotus ostreatus using solid state fermentation. Factorial design was used to study the effect of several nutrients on enzyme production. Purification and characterization of the enzyme and the effect of temperature, pH and gamma radiation on fungal growth and enzyme production was investigated. Optimization o...

In this work, a gene (lacP83) encoding a Pleurotus ostreatus laccase isoenzyme expressed in submerged fermentation conditions is described. A 2,887 bp sequence was obtained from a genomic library of P. ostreatus by using a PCR inverse strategy. The coding sequence, 1,527 bp long, showed 17 exons and encoded a protein of 509 amino acids, with a putative signal peptide and conserved copper bindin...

Pleurotus ostreatus better known as oyster mushroom is widely cultivated and consumed as food in Malaysia. The present study aims to assess the antioxidative potential and total phenolic content of P. ostreatus aqueous extract. The antioxidant activities were evaluated against DPPH and ABTS radical-scavenging activity, ferric-reducing antioxidant power (FRAP) and β-carotene-linoleate bleaching ...

The heterologous expression of the gene LACP83 (encoding a laccase) from Pleurotus ostreatus in Escherichia coli was characterized. The laccase enzyme activity and kinetics of bacterial growth with an inducer (IPTG) and without inducer were determined. The maximum enzymatic activity was observed at 7 h post induction with a value of 3740 ± 342 U/L, which was similar to that reported for the nat...

Biodegradation of polycyclic aromatic hydrocarbons (PAHs) in crude oil, by a white rot fungus, Pleurotus ostreatus sp., in broth culture was investigated. It was found that the biomass of the organism increased with the increase of PAHs concentration in the cultures. In the cultures with 25.99, 49.01 and 80.05 mg.kg-1 PAHs, the degradation increased with the PAHs of low molecular weight concent...

efficiency of pleurotus ostreatus and p. sajor-caju as biocontrol agents of the root knot nematode, meloidogyne javanica, was evaluated in vitro and in greenhouse experiments. population of the nematode were prepared and nematicidal effects of culture filtrates of the fungal specieson second stage juveniles of m. javanica were tested. substrates completely colonized by tested fungi as fungal in...