Background: The importance of plasmid-encoded Extended Spectrum Beta Lactamase (ESBL) organisms in the dissemination multi-drug resistance and complicated infections are contributory factors to treatment failure. Despite these, there is a paucity information regarding plasmid-borne associated infection from primary sources including cockroaches while studies correlating total protein ESBL also ...

ABSTRACT Plasmids are common in natural bacterial communities, facilitating evolution via horizontal gene transfer. Bacterial species vary their proficiency to host plasmids: whereas plasmids stably maintained some regardless of selection for plasmid-encoded genes, other species, even beneficial rapidly lost. It is, however, unclear how this variation affects plasmid persistence communities. He...

Some years ago, we found that Axial organ (AO) cells exerted an induced and spontaneous cytotoxicity against SP2 MBL2 mouse tumoral cells. Recently, discovered a sea star Igkappa gene with immune properties. This was first, inserted in CMV (cytomegalovirus) finally plasmid called « young » plasmid. In the present experiment IGKappa cloned HeK human to produce specific protein. The induced« prot...

Introduction: Multiple sclerosis (MS) is a disease of the immune system: it attacks the myelin around the axons and leaves them exposed. Destruction of myelin weakens the electrical conduction of ions and thus leads to a lack of communication in the nervous system.  Methods: In the present study, we constructed recombinant plasmid and then transformed to E. coli cell. The colonies containing...

Nonpolar virB mutants of Agrobacterium tumefaciens were tested for RSF1010 mobilization and extracellular complementation. virB2 to virB11 were essential for transfer in both assays. virB1 was essential only for high frequency transfer of RSF1010 and VirE2. Coordinated transfer of a preassembled T complex is supported by these data and competition studies.

Colistin is one of the last-resort therapeutic antibiotics used to control carbapenem-resistant Klebsiella pneumoniae. However, emergence colistin-resistant strains with high mortality and morbidity has become a new challenge. Therefore, it crucial characterize genotypic features carbapenem- K. pneumoniae (CCKP) isolates implement public health intervention strategies. In this study, we analyze...

conclusions the pc-ns3 possesses the capacity to express ns3 in the mammalian cell line and demonstrated strong immunogenicity in a murine model. our primary results demonstrated that the immunogenic truncated region of ns3 could be used as a potential vaccine candidate against hepatitis c. results the pcdna3.1 plasmid harboring the coding sequence of ns3 (pc-ns3) was constructed and confirmed ...

taq dna polymerase is widely used in laboratories and for this reason many investigators have focused their attention on understanding the role of various regions and amino acids in this enzyme. o-helix is a part of taq polymerase suggested to play an important role in the enzyme fidelity. the influence of asn666 in this helix on the enzyme function has never been investigated, and therefore by...