Two classes of nascent lipoproteins can be isolated from Golgi apparatus-rich fractions of liver from hypercholesterolemic rats. Golgi very low density lipoproteins (VLDL, d less than 1.006 g/ml) are enriched in cholesteryl esters and are similar in many respects to hypercholesterolemic plasma B-VLDL. Golgi low density lipoproteins (LDL, d 1.006-1.05 g/ml) are cholesteryl ester-rich beta-migrat...

Plasma lipoproteins of d<1.006 g/ml, d 1.006-1.019 g/ml, and d 1.019-1.063 g/ml from patients with familial lecithin:cholesterol acyltransferase deficiency yielded abnormal subfractions upon being separately filtered through 2% agarose gel. A subfraction that emerged with the void volume and contained unusually large amounts of unesterified cholesterol and phosphatidylcholine was present in eac...

Plasma lipoproteins from patients with familial lecithin:cholesterol acyltransferase (LCAT) deficiency have been fractioned by preparative ultra-centrifugation and gel filtration and their lipid content and reactivity studied. All of the lipoproteins are abnormal with respect to lipid concentration or relative lipid content. The low density lipoproteins (LDL) and high density lipoproteins (HDL)...

A method to measure apolipoprotein B radioactivity in whole blood plasma is described that is suitable for routine use in kinetic experiments in vivo. Radiolabeled apolipoprotein B is precipitated from plasma diluted 15- to 30-fold in the presence of carrier low density lipoproteins by 50% isopropanol. The amount of radioiodine in apoB is estimated from the difference between total radioiodine ...

In diabetic hypercholesterolemic rabbits at plasma triglyceride concentrations of approximately 5000 mg/dl, 55% of plasma cholesterol (1400 mg/dl) was in lipoproteins with diameters larger than 75 nm (Sf greater than 400), 40% in smaller very low density and intermediate density lipoproteins, 4% in low density lipoproteins, and 1% in high density lipoproteins. Specific intimal clearance (nl/h.m...

Vesicular lipoproteins (e.g., lipoprotein-X) are found in plasma in cholestasis or following infusion of Intralipid or phospholipid. To investigate the metabolism of vesicular lipoproteins, we isolated them from the plasma of subjects with cholestasis or following chronic or single Intralipid infusion. Cholestasis and chronic Intralipid therapy were found to be associated with elevated plasma c...

objective(s) the purpose of the present study was to evaluate the effect of ramadan fasting and weight-lifting training on plasma volume, glucose, and lipids profile of male weight-lifter. materials and methods forty male weight-lifters were recruited and divided into 4 groups (n=10 each) and as the following groups: control (c), fasting (f), training (t) and fasting-training (f-t). the t and f...

The intestine has long been recognized as n organ active in lipoprotein formation. This is hardly surprising when one considers the large quantities of dietary and endogenous lipids that traverse the intestinal mucosa daily and are absorbed into the mesenteric lymph as lipoproteins. Until recently, the major focus of research on intestinal ipoprotein metabolism was concerned with the intesti...

The dynamics of human apoE metabolism were explored by examining the effects of alimentary lipemia and postheparin lipolysis on the plasma level and lipoprotein distribution of apoE. In the studies of alimentary lipemia, fasting and postprandial plasma samples were obtained from five normal adult males, each of whom drank 100 g of corn oil. Although no change in the plasma concentration of apoE...