plant tissue culture techniques are used as basic requirement of common plant transformation systems. in most cases of plant transformation, a reproducible regeneration protocol is the limiting step due to long time lasting, specialized facilities and well experienced persons. furthermore, tissue culture procedures induce somaclonal variation among regenerated transgenic plants. therefore, rece...

Cell walls are part of the apoplasm pathway that transports water, solutes, and nutrients to cells within plant tissue. Pressures within the apoplasm (cell walls and xylem) are often different from atmospheric pressure during expansive growth of plant cells in tissue. The previously established Augmented Growth Equations are modified to evaluate the turgor pressure, water uptake, and expansive ...

Plant tissue culture, or the aseptic culture of cells, tissues, organs, and their components under defined physical and chemical conditions in vitro, is an important tool in both basic and applied studies as well as in commercial application. It owes its origin to the ideas of the German scientist, Haberlandt, at the beginning of the twentieth century. The early studies led to root cultures, em...

Plants are not only important producers of foods and energy storages (e.g., sugars, carbohydrates, proteins, and fats) in the form of grains, fruits, and vegetables, they also provide many valuable products to human existence including wood, fibers, oils, resins, pigments, antioxidants, and sources of medicine. Most importantly in light of this book, plants have been a source of therapeutic and...

In recent years, the possibility of developing numerous plantlets of many plant species in vitro has been realized. Termed plant tissue culture, this method of plant propagation has not been without problems when employed in commercial practice. One problem of major consequence has been bacterial contamination, usually becoming evident during later stages of tissue culture increase and multipli...

CONTENTS History of Plant Tissue Culture and Biotechnology Scope and Importance of Biotechnology Tissue and Cell Culture Micropropagation Anther Culture Production of Secondary Metabolites Protoplasts Isolation and Fusion Somatic Hybrids Somaclonal Variation Germplasm Conservation Genetic Engineering and Gene Technology Methods for Gene Transfer Generation of Transgenic Plants and their identif...

In a recent investigation1 a procedure was found that permitted fairly accurate determinations of the pH of soils of low moisture percentages. One of the essentials of the method was the use of a horn spoon in spreading the moisture or soil films uniformly throughout the rather dry soil mass. When plant tissue is finely cut in a grinder, relatively few of the cells are actually opened. The impo...

Plants or plant cells in vitro, show physiological and morphological response to microbial, physical or chemical factors which are known as ‘elicitors’. Elicitation is a process of induced or enhanced synthesis of secondary metabolites by the plants to ensure their survival persistence and competitiveness. The application of elicitors, which is currently the focus of research, has been consider...

History of Plant Tissue Culture and Biotechnology Scope and Importance of Biotechnology Tissue and Cell Culture Micropropagation Anther Culture Production of Secondary Metabolites Protoplasts Isolation and Fusion Somatic Hybrids Somaclonal Variation Germplasm Conservation Genetic Engineering and Gene Technology Methods for Gene Transfer Generation of Transgenic Plants and their identification M...