An efficient plant regeneration protocol was developed from leaf and internode (stem) explants of Bacopa monniera L. on MS basal medium, supplemented with Cytokinins like BAP, KN, and TDZ used singly. The leaf cultures expressed maximum plant regeneration potential with 138.9 ± 0.50 shoots per explants on MS + 2.0 mg/ l BAP. The in vitro regenerated dwarf shoots were further elongated on MS med...

Recent studies have provided significant new insights into the gene actions that specify and maintain stem cells in plant shoots and roots. New layers of genetic control and potential signalling pathways and effector mechanisms have emerged from these new studies and will be reviewed here. These new findings refine the current model in which stem cells in plant meristems are regulated by negati...

Quercetin is known to possess antimicrobial activity against bacteria, fungi, and viruses. The activity of this flavonoid against phytoplasmas, non-cultivable plant pathogenic bacteria that cause numerous plant diseases, has never been examined before. The aim of this research was to examine the effect of different concentrations of quercetin (10 mM, 100 mM and 1 mM) on 'Candidatus Phytoplasma ...

An efficient plant regeneration protocol has been developed from leaf explants of Stevia rebaudiana, an economically and medicinally important herbaceous plant species belonging to the family Asteraceae. Plant regeneration and multiple shoot proliferation was initiated from leaf explants cultured on MS medium supplemented with various concentrations of 6-benzyladenine (BA) and kinetin (KN) in c...

The presence of plant-uncoupling mitochondrial protein (PUMP), previously described by Vercesi et al. (1995), was screened in mitochondria of various organs or tissues of several plant species. This was done functionally, by monitoring purine nucleotide-sensitive linoleic acid-induced uncoupling, or by Western blots. The following findings were established: (1) PUMP was found in most of the hig...

An efficient in vitro plant regeneration protocol was developed for Chrysanthemum morifolium, an ornamental plant. Nodal segments were used as explants in the present experiment. The explants were cultured onto Murashige & Skoog’s (MS) nutrient medium containing plant growth regulators (cytokinins or auxins) with various combinations and concentrations for the study. Maximum percentage (93.33%)...

An efficient in vitro regeneration protocol has been developed from shoot tips of Drosera burmannii Vahl., a carnivorous plant of north-east India. Various plant growth regulators were used to study their efficacy in the induction of multiple shoots and roots. Of the various treatments, the maximum number of shoots (28.8 ± 1.5) and roots (9.7 ± 0.6) was observed in one-fourth strength standard ...

Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supplemented with BAP (0.44-2.22 microM) showed production of de novo shoots. Roots attached to mother plant showed more number of shoots, while excised root segments produced lesser shoots. Shoots (0.5-0.8 cm) were transferred to elongation medium consisting of Woody Plant Medium (WPM) supplemented wit...

1.Labelling plants with 15N and 13C stable isotopes usually require cultivation of plants in isotopically enriched soil and gas-tight labelling chambers - both approaches are not suitable if one aims to investigate in situ species interactions in real plant communities. 2.In this greenhouse experiment, we tested a labelling method in which dual-labelled (15N, 13C) urea solution is brushed direc...

Uptakes of o-chlorophenol (CP), 2,4-dichlorophenol (DCP), trichloroethylene (TCE), and atrazine (ATR), as single and mixed contaminants, by roots and shoots of rice seedlings (Oryza sativa L.) from hydroponic solutions were measured following a 48-h exposure of plant roots. As single contaminants, the concentrations of CP, DCP, and ATR in rice roots and shoots increased significantly with incre...