Alkaline protease producing microorganism Vibrio metschnikovii NG155, isolated from the soil samples of leather industry, having potential application in dehairing of animal skin was used in this study. To enhance the production of protease by this strain, various physical and nutritional factors were optimized using statistical methods like Plackett Burman and Response Surface Methodology. In ...

Article history: Received on: 05/10/2015 Revised on: 23/10/2015 Accepted on: 08/11/2015 Available online: 27/12/2015 This work, for the first time reports the optimization of recombinant human antithrombin (rhAT) production in Saccharomyces cerevisiae BY4741 using statistical experimental designs. Most applications of design of experiments (DoE) have concerned optimization of the composition of...

A novel expression vector constructed from genes of Pichia pastoris was applied for heterologous gene expression in Saccharomyces cerevisiae. Recombinant streptokinase (SK) was synthesized by cloning the region encoding mature SK under the control of glyceraldehyde 3-phosphate dehydrogenase (GAP) promoter of Pichia pastoris in Saccharomyces cerevisiae. SK was intracellularly expressed constitut...

Plackett-Burman design, central composite design and response surface analysis were carried out with the aim of optimizing culture conditions for -mannanase production from Bacillus licheniformis TJ-101. Screening experiments of Plackett-Burman design were firstly employed to evaluate the effects of 16 variables on -mannanase production. The four identified significant variables, i.e. the conce...

This is an "11 factor-2 level-12 run" Plackett-Burman experimental design dataset. The dataset includes 11 engineering bioreactor parameters as input variables. These 11 factors were varied at 2 levels and 23 response variables that are glycan profile attributes, were measured "A Design Space Exploration for Control of Critical Quality Attributes of mAb" (H. Bhatia, E.K. Read, C.D. Agarabi, K.A...

Response surface methodology was applied to optimize medium constituents for enzymatic production of L-cysteine from DL-2-amino-D-thiazoline-4-carboxylic acid (DL-ATC) by a novel Pseudomonas sp. Zjwp-14. With the Plackett-Burman design experiment, glycerol, DL-ATC, yeast extract, and pH were found to be the most powerful factors among the eight tested variables that influence intracellular enzy...

Nonregular two-level designs are attractive screening due to their good projection properties and flexible run sizes. In particular, the 12-run Plackett–Burman (PB) design has become quite popular. However, existing methods struggle with identification of active factors when number exceeds projectivity designs. This is especially case interactions present, variance high runs small. this paper, ...