Plackett-Burman design, central composite design and response surface analysis were carried out with the aim of optimizing culture conditions for -mannanase production from Bacillus licheniformis TJ-101. Screening experiments of Plackett-Burman design were firstly employed to evaluate the effects of 16 variables on -mannanase production. The four identified significant variables, i.e. the conce...

This is an "11 factor-2 level-12 run" Plackett-Burman experimental design dataset. The dataset includes 11 engineering bioreactor parameters as input variables. These 11 factors were varied at 2 levels and 23 response variables that are glycan profile attributes, were measured "A Design Space Exploration for Control of Critical Quality Attributes of mAb" (H. Bhatia, E.K. Read, C.D. Agarabi, K.A...

Response surface methodology was applied to optimize medium constituents for enzymatic production of L-cysteine from DL-2-amino-D-thiazoline-4-carboxylic acid (DL-ATC) by a novel Pseudomonas sp. Zjwp-14. With the Plackett-Burman design experiment, glycerol, DL-ATC, yeast extract, and pH were found to be the most powerful factors among the eight tested variables that influence intracellular enzy...

234 Application of Statistical Design to Optimize Culture Medium for Xylanase Production by Bacillus pumilus AB-1 Snehal Ingale, Sonam Kalyani 1 and Urvish Chhaya Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology & Allied Sciences, New Vallabh Vidyanagar-388120, Gujarat, India Natubhai V. Patel College of Pure and Applied Sciences, Vallabh Vidyanagar-388120, Gujar...

Methotrexate (MTX) being the widely used biologic agent is used to treat severe and/or disabling psoriasis but precipitates various side effects on oral therapy. Encapsulating methotrexate in flexible liposomes for delivery through skin could lead to better therapeutic action and reduce side effects. In order to screen out the various factors during liposome formulation development, Plackett Bu...

A stability-indicating liquid chromatography method for the determination of the antifungal agent butenafine hydrochloride (BTF) in a cream was developed and validated using the Plackett-Burman experimental design for robustness evaluation. Also, the drug photodegradation kinetics was determined. The analytical column was operated with acetonitrile, methanol and a solution of triethylamine 0.3%...

Marine actinomycetes were isolated from sediment samples collected from Pitchavaram mangrove ecosystem situated along the southeast coast of India. Maximum actinomycete population was noted in rhizosphere region. About 38% of the isolates produced L-asparaginase. One potential strain KUA106 produced higher level of enzyme using tryptone glucose yeast extract medium. Based on the studied phenoty...