UTILITY: (1) Assess degree of fibrosis. H&E stain is not reliable for demonstration of fibrosis especially in early stages. Pericellular fibrosis around the central vein in steatohepatitis can be difficult to identify on the H&E stain. (2) Distinguish fibrosis from necrosis. In acute hepatitis, areas of confluent necrosis can be difficult to distinguish from fibrosis and can lead to an erroneou...

Clinical isolates of the opportunistic pathogen Mycobacterium avium complex (MAC) undergo a reversible switch between red and white colony morphotypes on agar plates containing the lipoprotein stain Congo red. Compared to their isogenic red counterparts, white morphotypic variants are more virulent and more resistant to multiple antibiotics. This report shows that the two-component regulatory s...

We report that the dye nile red, 9-diethylamino-5H-benzo[alpha]phenoxazine-5-one, is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. The specificity of the dye for lipid droplets was assessed on cultured aortic smooth muscle cells and on cultured peritoneal macrophages that were incubated with acetylated low density...

BACKGROUND Breast carcinoma in situ (CIS) is classified into ductal carcinoma in situ (DCIS) and lobular carcinoma in situ (LCIS). DCIS is treated with surgical excision while LCIS can be clinically followed with or without hormonal treatment. Thus, it is critical to distinguish DCIS from LCIS. Immunohistochemical (IHC) staining for E-cadherin is routinely used to differentiate DCIS from LCIS i...

statement of problem: primary teeth discoloration can cause mentally and socially unpleasant effects in children. this can also be a sign of systemic disease. despite the importance of this subject, a comprehensive study has not been performed yet. purpose: the aim of this study was determining the frequency of different types of primary teeth discoloration. materials and methods: in this cross...

This communication reports the use of Congo red to stain the nanofibers of self-assembled small molecules for assaying intracellular supramolecular hydrogels, which provides a convenient method to explore molecular self-assembly inside cells.

To obtain as much information as possible from light-microscopy about changes in the glomerular basement-membrane, a thin section microtome attachment is necessary in order to produce 1 to 3 micron sections. The most informative staining method of paraffin embedded tissue is the Muller-Mowry-Masson trichrome (M-M-M) combination stain. The deposits, e.g., in systemic lupus erythematosus (SLE), s...