YM-47522, a novel antibiotic, was isolated from the culture broth of Bacillus sp. YL-03709B. The antibiotic was purified by centrifugal partition chromatography and ODS column chromatography. It exhibited potent in vitro antifungal activity especially against Rhodotorula acuta and Pichia angusta (MIC: 0.05 and 0.75 microgram/ml, respectively). It also showed moderate or weak antifungal activity...

Promoters are indispensable elements of a standardized parts collection for synthetic biology. Regulated promoters of a wide variety of well-defined induction ratios and expression strengths are highly interesting for many applications. Exemplarily, we discuss the application of published genome scale transcriptomics data for the primary selection of methanol inducible promoters of the yeast Pi...

A gene encoding Rhizopus oryzae lipase containing prosequence (ProROL) was cloned into the pPICZαA and electrotransformed into the Pichia pastoris X-33 strain. The lipase was functionally expressed and secreted in Pichia pastoris with a molecular weight of 35 kDa. The maximum lipase activity of recombinant lipase (rProROL) was 21,000 U/mL, which was obtained in a fed-batch cultivation after 168...

the aim of this study was to evaluate the performances of methods such as sequencing of the internal transcribed spacer (its) and 26s (d1/d2) regions of ribosomal dna, rflp analysis of the its region and commercial biochemical test kit for the identification of the yeasts isolated during spontaneous fermentation of fresh crushed pineapple juice. the experiments were conducted in thailand and au...

In the course of studying the taxonomic diversity of osmo-tolerant yeasts in Tropical Southeast Asia, many yeast strains have been isolated from traditional fermented food, fruits, and other natural habitats. Among our isolates several new species were predicted by 26S rDNA D1/D2 nucleotide sequence analysis and a cation-tolerance test using LiCl (Nagatsuka et al., 2002, 2005). Citeromyces siam...

Oryctes rhinoceros merupakan serangga fitofag dan telah dilaporkan sebagai hama di beberapa negara. Larva O. rinocheros makan pada bahan organik (bagian tanaman yang membusuk) tidak menyebabkan kerusakan ekonomi. Seperti kebanyakan serangga, berinteraksi dengan mikroorganisme sistem pencernaanya. Selain itu, dalam saluran pencernaan juga sumber daya alam bermanfaat untuk kepentingan manusia, se...