The potential for using Pichia guilliermondii BCC 5389 or Bacillus subtilis ABS-S14 by themselves or in combination for the control Penicillium digitatum in citrus, and their effects on postharvest quality of fruit was investigated. The percentage of disease with the combined antagonists was completely inhibited. Rapid colonization of P. guilliermondii was observed in the wounds during the firs...

In previous studies it was shown that Pichia guilliermondii strain Z1, isolated from healthy Moroccan citrus Valencia-Late oranges, was effective against Penicillium italicum. Here the effectiveness of strain Z1 was assessed against Penicillium digitatum, the causal agent of green mould, under different temperature (5-25°C) and relative humidity (RH) (45-100%) regimes for its reliable and large...

The performance of the Etest for posaconazole (SCH 56592) susceptibility testing of 314 isolates of Candida spp. was assessed against the National Committee for Clinical Laboratory Standards (NCCLS) microdilution broth method. The NCCLS method employed RPMI 1640 broth medium, and MICs were read after incubation for 48 h at 35 degrees C. MICs were determined by Etest for all 314 isolates with RP...

Twenty-five yeast cultures, mainly of human origin, belonging to four pathogenic yeast species--Candida albicans, Candida krusei, Candida parapsilosis, and Candida tropicalis were tested for their sensitivity to ten basidiomycetous and eleven ascomycetous yeast species isolated from the water and soil environments and from tree leaves. The best killer activity among basidiomycetous species was ...

Debaryomyces hansenii is a hemiascomycetous yeast commonly found in natural substrates and in various types of cheese. Pichia guilliermondii is widely distributed in nature and is a common constituent of the normal human microflora. Both species have been described in human infections but are extremely difficult to differentiate phenotypically. Thus, frequent errors in identification occur. The...

The effects of yeast extract (0-10 g/l), methanol (0-10% v/v), acetic acid (0-1.0 g/l), furfural (0-0.5 g/l), glucose (0-30 g/l), inoculum age (15-70 h) and product concentration (18-230 g/l) on the xylose-xylitol conversion by Candida guilliermondii FTI 20037 were studied. The xylitol specific productivity increased about 35% at a yeast extract concentration of 1.0 g/l, whereas glucose showed ...

Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation of these species. Our assay was vali...

It has been confirmed that Saccharomyces sp. W0 can produce high concentration of ethanol. In this study, the INU1 gene cloned from the marine-derived Pichia guilliermondii was transformed into uracil mutant of Saccharomyces sp. W0. The positive transformant Inu-66 obtained could produce 34.2 U ml⁻¹ of extracellular inulinase within 72 h of cultivation. It was found that 15.2 U of inulinase act...