Several segments (∼20  kbp) of the lime witches' broom (LWB) phytoplasma genome (16SrII group) were sequenced and analysed. A 5.7  kbp segment (LWB-C) included conserved genes whose phylogenetic tree was consistent with that generated using 16S rRNA genes. Another 6.4  kbp LWB phytoplasma genome segment (LWB-NC) was structurally similar to the putative mobile unit or sequence variable mosaic ge...

The chromosome sequence of "Candidatus Phytoplasma australiense" (subgroup tuf-Australia I; rp-A), associated with dieback in papaya, Australian grapevine yellows in grapevine, and several other important plant diseases, was determined. The circular chromosome is represented by 879,324 nucleotides, a GC content of 27%, and 839 protein-coding genes. Five hundred two of these protein-coding genes...

A 927 bp non-ribosomal fragment was used to assess the genetic variability of the European stone fruit yellows (ESFY) phytoplasma infecting 14 different Prunus species. For this, 175 isolates originating from four different Mediterranean countries were tested by PCR-RFLP analysis with seven restriction enzymes. No polymorphism among the ESFY phytoplasma could be observed but 12 out of 18 restri...

Individual lines of Vitis vinifera Barbera and Chardonnay were obtained by in vitro culture of axillary buds collected from phytoplasma-affected mother plants. The molecular assays did not detect the “flavescence dorée” (FD) phytoplasma in the 66 lines tested after 6-9 months of culture, while the “bois noir”-associated phytoplasma was detected in 22 out of 50 lines. Despite the observed ineffi...

The base map of domains was drawn using NCBI Conserved Domains searches (http://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi). Rep_2, Plasmid replication protein (pfam01719), Viral_Rep, Putative viral replication protein (pfam02407); Gemini_AL1, Geminivirus Rep catalytic domain (pfam00799); Gemini_AL1_M, Geminivirus rep protein central domain (pfam08283); RNA_helicase, RNA helicase (pfam00910)....

Potato purple top (PPT) is an emerging potato disease complex. The etiological agents of PPT are phloem-inhabiting, cell wallless bacteria known as phytoplasmas. Tomato is an alternative host of these phytoplasmas. PPT phytoplasma-infected tomato plants exhibit symptoms including abnormal foliage development and formation of “big buds”. In the present study, twodimensional gel electrophoresis (...

This study is the first to report a phytoplasma associated with machorreo in lulo. We detected the presence of a phytoplasma in association with machorreo in lulo, using phylogenetic analysis and homology with the sequence of the 16S rRNA region and the gene tRNA. We identified the phytoplasma as belonging to the 16Sr III group (Xdisease; 97% homology). For future studies, we recommend identify...

In September 2007, a peach tree (Prunus persica) displaying yellowing symptoms indicative of phytoplasma infection was sampled in Quba region of Azerbaijan. A phytoplasma was detected in the diseased peach tree by nested PCR amplification of its 16Sr DNA with universal primers for phytoplasmas. Phylogenetic analyses of the amplified 16S rDNA showed that the phytoplasma infecting peach tree corr...

Bois noir (BN) is an insect-transmitted grapevine yellows disease caused by phytoplasmas belonging to the stolbur subgroup 16SrXII-A. In Italy, increasing prevalence of stolbur phytoplasma strains in vineyards suggests progressive spread of the disease and potential for heavy impacts on the wine industry. In this study, we investigated the genetic diversity of stolbur phytoplasma strains in BN ...

in 2011-2012 surveys for phytoplasma diseases, eggplant )solanum melongena l.( plants with phyllody symptoms were observed in eggplant fields of roodan (hormozgan province of iran). agent of roodan eggplant phyllody (rep) was transmitted from phyllody affected eggplant to eggplant and tomato by grafting and to periwinkle via dodder inoculation inducing phytoplasma-type symptoms. phytoplasmal in...