It is proposed that enzymic phosphoryl-transfer reactions occur by concerted, step-wise, associative (phosphorane-intermediate) or dissociative (metaphosphate-intermediate) mechanisms, as dictated by the catalytic scaffold and the reactants. During the evolution of a phosphotransferase family, the mechanism of the phosphoryl-transfer reaction is in constant flux, potentially changing with each ...

The variable mechanism for substitution of P-stereogenic phosphoryl chloride with alkynyl metallic reagents, which depends on temperature, stoichiometry of starting materials, and the structure of the nucleophilic reagent, is assumed as either SN2-like or Berry pseudorotation of pentacoordinated phosphorus intermediates, affording inversion and retention products, respectively. The formation of...

Accepted on 26 th December 2016, Published online on 27 th January 2017 _____________________________________________________________________________ ABSTRACT During the present investigation, a new series of 3,6-disubstituted[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole derivatives (3) were synthesized by refluxing a mixture of 3-substituted-4-amino-5-mercapto-1,2,4triazoles with substituted anilin...

Rabbit muscle phosphoglucomutase converts a-D-glucose 1_[(S)-160,17O,8Olphosphate into D-glucose 6-[160,'70,18Olphosphate, which is shown by 31P n.m.r. spectroscopy, after cyclization and methylation, to have the (S)-configuration at the phosphorus atom. Since phosphoglucomutase is known to catalyse phosphoryl transfer by way of a phospho-enzyme intermediate, and since individual phosphoryl-tra...

Purified phosphoryl transferase catalyzes an ATP-ADP exchange reaction. Evidence is presented which excludes the possibility that the exchange activity of the preparation is due to contaminating adenylate kinase. The rate of the exchange reaction is affected by the molar ratio of ATP to ADP. The reaction is not highly specific either for the divalent metal ion or for the nucleoside triphosphate...

Phosphoryl transfer from the high-energy phosphate donor ATP is ubiquitous in biological chemistry, and nitrogen nucleophiles, especially histidine, often serve as in vivo acceptors of the γ-phosphate of ATP. Nevertheless, nonenzymatic reactions of ATP with amines have not previously been characterized. We have therefore examined phosphoryl transfer from ATP to amines to provide a basis for und...

Reversible phosphorylation of proteins is a post-translational modification that regulates diverse biological processes. The molecular mechanism underlying phosphoryl transfer catalyzed by enzymes, in particular the nature of transition state (TS), remains a subject of active debate. Structural evidence supports an associative TS, whereas physical organic studies point to a dissociative charact...

nucleotide was increased, and, assuming that the product was derived from oligonucleotides and deoxyribonucleoside triphosphates, the synthesis amounted to 74% of the deoxyribonucleic acid primer. 4. The nucleotidyltransferase was inhibited and the lag phase extended by 3'-phosphoryl-terminal oligonucleotides from a splenic-deoxyribonuclease digest of deoxyribonucleic acid. 5. The compounds obt...

The phosphoryl group, PO3-, is the dynamic structural unit in the biological chemistry of phosphorus. Its transfer from a donor to an acceptor atom, with oxygen much more prevalent than nitrogen, carbon, or sulfur, is at the core of a great majority of enzyme-catalyzed reactions involving phosphate esters, anhydrides, amidates, and phosphorothioates. The serendipitous discovery that the phospho...

Quinacridone chemistry has been developed which provides a source of new compounds that have potential application in organic photovoltaic (OPV) devices. Phosphoryl chloride is used for the conversion carbonyl groups N,N′-dialkylquinacridones, generating reactive intermediates enable selective condensation with either one or two nucleophilic molecules under mild conditions.