Background and Purpose: Today, treatment of life-threatening fungal infections, caused by Candida species, has become a major problem. In the present study, we aimed to evaluate the antifungal susceptibility patterns of different clinical Candida isolates, determine the virulence factors in multi-drug resistant (MDR) Candida species, and assess the anti-biofilm activity of Elettaria cardamomum ...

The structure and function of the platelet surface was probed by phospholipase C (Clostridium perfringens) which hydrolyzes membrane phospholipids, particularly phosphatidylcholine. Platelet phospholipids were susceptible to phospholipase C, and extent of hydrolysis was dependent on concentration of phospholipase C and Ca(++). Phospholipase C (0.15 U/ml) with Ca(++) (0.55 mM) hydrolyzed 15.6% p...

Eighty-three percent of polyphosphoinositide-specific phospholipase C activity was recovered in a cytosolic fraction after nitrogen cavitation of turkey erythrocytes. This activity has been purified approximately 50,000-fold when compared to the starting cytosol with a yield of 1.7-5.0%. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the phospholipase C preparation revealed a majo...

To explore the roles of vascular phospholipase C activity in the development of hypertension, phospholipase C activity was examined in the aortic wall of spontaneously hypertensive rats (SHR). Phospholipase C activity was significantly enhanced (+87%, p less than 0.005) in 14-week-old SHR as compared with normotensive Wistar-Kyoto rats (WKY). The enzymatic activities were positively correlated ...

The role of phospholipid in rat liver microsomal glucose 6phosphatase has been investigated with the use of phospholipase A and phospholipase C to alter the microsomal phospholipids. The phospholipase C-treated preparation lost a maximum of 80 to 90% of the original activity, and 70% of the microsomal phospholipid was hydrolyzed. Addition of phospholipid completely restored the original activit...

Background: Candida species is a normal commensal flora of human body inhabiting the skin, mucous membrane and gastro intestinal tract but may be associated with superficial deep-seated fungal infections. The switch from to potent pathogen, facilitated by various extracellular hydrolytic enzymes. aim this study was estimate phospholipase, proteinase, haemolysin esterase activity determine antif...