Spectrin was shown previously to interact with phosphatidylserine and phosphatidylethanolamine. which are preferentially localized in the inner half of the membrane lipid bilayer. but this interaction is not well characterized. In the present study we used electron microscopy of rotaryshadowed platinum replicas of spectrin dimer-phosphatidylserine complexes to study the interaction of spectrin ...

Received December 5,1979 SUMMARY The influence of divalent cations, and pH on the behaviour of phosphatidylserine, derived from egg phosphatidylcholine, has been examined employing 31P-NMR techniques. The addition of Ca2+ results in the observation of a "rigid lattice" 31P-NMR spectra and more than an order of magnitude increase in the spin-lattice relaxation time Tl. specific headgroup immobil...

Glucose depletion of erythrocytes triggers suicidal erythrocyte death or eryptosis, which leads to cell membrane scrambling with phosphatidylserine exposure at the cell surface. Eryptotic erythrocytes adhere to endothelial cells by a mechanism involving phosphatidylserine at the erythrocyte surface and CXCL16 as well as CD36 at the endothelial cell membrane. Nothing has hitherto been known abou...

An approach to the large-scale preparation of highly pure phosphatidylserine from bovine brain is described in this paper. The method is based on (i) the separation of phosphatidylserine from phosphatidylinositol in bovine brain extract by preparative aminopropyl normal-phase high-performance liquid chromatography using methanol-1 M phosphoric acid (90:10, v/v) as mobile phase and (ii) further ...

Phosphatidylserine exposure in platelets is required for normal haemostasis and is also a hallmark of apoptosis. It results from activation of a phospholipid scramblase, which has been shown to be differently stimulated by Ca(2+)-influx and during apoptosis, thus suggesting that mitochondria may be involved in phosphatidylserine exposure in platelets. It is also well known that local anaestheti...

CDP-diglyceride: L-serine phosphatidyltransferase (phosphatidylserine synthetase) of Escherichia coli is tightly associated with ribosomes in crude cell-free extracts. The synthetase has now been separated from ribosomes by extraction with solutions containing 5 M NaCl and has been purified loo-fold. The partially purified enzyme is devoid of contaminating hydrolytic activities and nearly free ...

Increased exposure of sickle red blood cells to phosphatidylserine promotes its adhesion to the endothelium. A monoclonal antibody to lactadherin, a phosphatidylserine binding protein, inhibits sickle cell adhesion to histamine-stimulated endothelial cells in flowing blood. Added lactadherin enhances the adhesion via the integrin alphaVbeta3. These results indicate that lactadherin can mediate ...

Phosphatidylserine on the dying cell surface helps identify apoptotic cells to phagocytes, which then engulf them. A candidate phagocyte receptor for phosphatidylserine was identified using phage display, but the phenotypes of knockout mice lacking this presumptive receptor, as well as the location of the protein within cells, cast doubt on the assignment of this protein as the phosphatidylseri...